Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins.

Published

Journal Article

Recently, a human cDNA clone with high sequence homology to the photolyase/blue-light photoreceptor family was identified. The putative protein encoded by this gene exhibited a strikingly high (48% identity) degree of homology to the Drosophila melanogaster (6-4) photolyase [Todo et al. (1996) Science 272, 109-112]. We have now identified a second human gene whose amino acid sequence displays 73% identity to the first one and have named the two genes CRY1 and CRY2, respectively. The corresponding proteins hCRY1 and hCRY2 were purified and characterized as maltose-binding fusion proteins. Similar to other members of the photolyase/blue-light photoreceptor family, both proteins were found to contain FAD and a pterin cofactor. Like the plant blue-light photoreceptors, both hCRY1 and hCRY2 lacked photolyase activity on the cyclobutane pyrimidine dimer and the (6-4) photoproduct. We conclude that these newly discovered members of the photolyase/photoreceptor family are not photolyases and instead may function as blue-light photoreceptors in humans.

Full Text

Duke Authors

Cited Authors

  • Hsu, DS; Zhao, X; Zhao, S; Kazantsev, A; Wang, RP; Todo, T; Wei, YF; Sancar, A

Published Date

  • November 1996

Published In

Volume / Issue

  • 35 / 44

Start / End Page

  • 13871 - 13877

PubMed ID

  • 8909283

Pubmed Central ID

  • 8909283

Electronic International Standard Serial Number (EISSN)

  • 1520-4995

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi962209o

Language

  • eng