Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins.
Journal Article (Journal Article)
Recently, a human cDNA clone with high sequence homology to the photolyase/blue-light photoreceptor family was identified. The putative protein encoded by this gene exhibited a strikingly high (48% identity) degree of homology to the Drosophila melanogaster (6-4) photolyase [Todo et al. (1996) Science 272, 109-112]. We have now identified a second human gene whose amino acid sequence displays 73% identity to the first one and have named the two genes CRY1 and CRY2, respectively. The corresponding proteins hCRY1 and hCRY2 were purified and characterized as maltose-binding fusion proteins. Similar to other members of the photolyase/blue-light photoreceptor family, both proteins were found to contain FAD and a pterin cofactor. Like the plant blue-light photoreceptors, both hCRY1 and hCRY2 lacked photolyase activity on the cyclobutane pyrimidine dimer and the (6-4) photoproduct. We conclude that these newly discovered members of the photolyase/photoreceptor family are not photolyases and instead may function as blue-light photoreceptors in humans.
Full Text
Duke Authors
Cited Authors
- Hsu, DS; Zhao, X; Zhao, S; Kazantsev, A; Wang, RP; Todo, T; Wei, YF; Sancar, A
Published Date
- November 5, 1996
Published In
Volume / Issue
- 35 / 44
Start / End Page
- 13871 - 13877
PubMed ID
- 8909283
International Standard Serial Number (ISSN)
- 0006-2960
Digital Object Identifier (DOI)
- 10.1021/bi962209o
Language
- eng
Conference Location
- United States