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Absence of phosphatidylinositol (PI)-linked proteins in a very early human multipotential haematopoietic marrow cell.

Publication ,  Journal Article
Huang, AT; Panella, TJ; Mold, NG; Rosse, WF
Published in: Br J Haematol
February 1991

A very early human haematopoietic progenitor cell population which was negative for the major histocompatibility class II antigen (HLA-DR) and positive for the CD34 (MY10) antigen was separated into two subsets according to the expression of decay-accelerating factor (DAF) on the cell surface. Using immunoadherence, cell cycle analysis, and cell culture, we determined that there is a DAF- multipotential cell and a more differentiated DAF+ lineage specific progenitor cell existing in human bone marrow. The DAF- subset was highly enriched for CFU-GEMM, while the DAF+ subset contained only BFU-E and CFU-GM. The DAF- subset was approximately 0.03% and the DAF+ subset approximately 0.008% of the original bone marrow population. MIRL (membrane-inhibitory of reactive lysis), another PI-linked protein, was not expressed on the DAF- population but was expressed on the DAF+ cells. These observations indicate that PI-linked proteins are absent from the multipotential stem cell but are present on an early lineage specific cell. The absence of expression of PI-linked proteins can be used to further isolate and characterize a very early multipotential haematopoietic progenitor cell population.

Duke Scholars

Published In

Br J Haematol

DOI

ISSN

0007-1048

Publication Date

February 1991

Volume

77

Issue

2

Start / End Page

145 / 149

Location

England

Related Subject Headings

  • Membrane Proteins
  • Immunology
  • Humans
  • Hematopoietic Stem Cells
  • Complement Inactivator Proteins
  • Colony-Forming Units Assay
  • Cells, Cultured
  • Cell Cycle
  • CD55 Antigens
  • Blood Proteins
 

Citation

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MLA
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Huang, A. T., Panella, T. J., Mold, N. G., & Rosse, W. F. (1991). Absence of phosphatidylinositol (PI)-linked proteins in a very early human multipotential haematopoietic marrow cell. Br J Haematol, 77(2), 145–149. https://doi.org/10.1111/j.1365-2141.1991.tb07969.x
Huang, A. T., T. J. Panella, N. G. Mold, and W. F. Rosse. “Absence of phosphatidylinositol (PI)-linked proteins in a very early human multipotential haematopoietic marrow cell.Br J Haematol 77, no. 2 (February 1991): 145–49. https://doi.org/10.1111/j.1365-2141.1991.tb07969.x.
Huang AT, Panella TJ, Mold NG, Rosse WF. Absence of phosphatidylinositol (PI)-linked proteins in a very early human multipotential haematopoietic marrow cell. Br J Haematol. 1991 Feb;77(2):145–9.
Huang, A. T., et al. “Absence of phosphatidylinositol (PI)-linked proteins in a very early human multipotential haematopoietic marrow cell.Br J Haematol, vol. 77, no. 2, Feb. 1991, pp. 145–49. Pubmed, doi:10.1111/j.1365-2141.1991.tb07969.x.
Huang AT, Panella TJ, Mold NG, Rosse WF. Absence of phosphatidylinositol (PI)-linked proteins in a very early human multipotential haematopoietic marrow cell. Br J Haematol. 1991 Feb;77(2):145–149.
Journal cover image

Published In

Br J Haematol

DOI

ISSN

0007-1048

Publication Date

February 1991

Volume

77

Issue

2

Start / End Page

145 / 149

Location

England

Related Subject Headings

  • Membrane Proteins
  • Immunology
  • Humans
  • Hematopoietic Stem Cells
  • Complement Inactivator Proteins
  • Colony-Forming Units Assay
  • Cells, Cultured
  • Cell Cycle
  • CD55 Antigens
  • Blood Proteins