Antithymocyte globulin stimulates human hematopoietic progenitor cells.

Published

Journal Article

Antithymocyte globulin (ATG), a horse antihuman thymus antiserum highly effective in the majority of patients with aplastic anemia, was studied for its in vitro effects on hematopoietic progenitor cells. Marrow cells isolated by an immunoadherence technique with the HPCA-1 (human progenitor cell antigen) monoclonal antibody after removal of contaminating T cells and macrophages formed erythroid colonies in methyl cellulose media in the presence of ATG at concentrations of 25-50 micrograms/ml. ATG also stimulated continuous production of hemoglobin-containing erythroid colonies beyond 35 days of culture when it was added to the culture weekly. ATG also had an indirect effect on myeloid (granulocytic and macrophagic) colony growth in vitro. At a concentration of 10 micrograms/ml, ATG stimulated late but not early myeloid progenitor cells to form mature colonies. This effect required the participation of lymphocytes containing the Leu-11 antigen and macrophages or supernatant fluids made from these two types of cells that had been preincubated with ATG for 3 hr and then cultured for 5 additional days. The supernatant fluids produced in such a manner showed characteristics similar to granulocyte colony-stimulating factor and had an activity peak that eluted at a volume corresponding to a 20,000 Da molecular mass protein by high-resolution liquid chromatography.

Full Text

Duke Authors

Cited Authors

  • Huang, AT; Mold, NG; Zhang, SF

Published Date

  • August 1987

Published In

Volume / Issue

  • 84 / 16

Start / End Page

  • 5942 - 5946

PubMed ID

  • 3112777

Pubmed Central ID

  • 3112777

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.84.16.5942

Language

  • eng

Conference Location

  • United States