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The use of in vitro technics to study drug-induced pancytopenia.

Publication ,  Journal Article
Kelton, JG; Huang, AT; Mold, N; Logue, G; Rosse, WF
Published in: N Engl J Med
September 20, 1979

The confirmation of drug-induced marrow aplasia is difficult since rechallenging the patient can lead to serious morbidity. We used marrow-culture technics to challenge in vitro the bone marrow of a patient with marrow aplasia after ingestion of quinidine. There was no clinical or laboratory evidence of quinidine-mediated destruction of erythrocytes, leukocytes or platelets. By contrast, use of quinidine in combination with the patient's serum substantially inhibited in vitro growth of allogeneic marrow granulocytic and erythroid series. Furthermore, use of quinidine in combination with acute-phase serum (but not acute-phase serum alone or quinidine in combination with recovery-phase serum) inhibited growth of the patient's marrow. This observation suggests that both a transient serum factor and quinidine were responsible for the marrow aplasia. These technics could be applied with minimal risk to similar patients and would permit in vitro rechallenge with the suspected drug.

Duke Scholars

Published In

N Engl J Med

DOI

ISSN

0028-4793

Publication Date

September 20, 1979

Volume

301

Issue

12

Start / End Page

621 / 624

Location

United States

Related Subject Headings

  • Quinidine
  • Pancytopenia
  • Methods
  • Male
  • Leukocytes
  • Immunoglobulin G
  • Humans
  • Hematopoietic Stem Cells
  • General & Internal Medicine
  • Erythrocytes
 

Citation

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ICMJE
MLA
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Kelton, J. G., Huang, A. T., Mold, N., Logue, G., & Rosse, W. F. (1979). The use of in vitro technics to study drug-induced pancytopenia. N Engl J Med, 301(12), 621–624. https://doi.org/10.1056/NEJM197909203011201
Kelton, J. G., A. T. Huang, N. Mold, G. Logue, and W. F. Rosse. “The use of in vitro technics to study drug-induced pancytopenia.N Engl J Med 301, no. 12 (September 20, 1979): 621–24. https://doi.org/10.1056/NEJM197909203011201.
Kelton JG, Huang AT, Mold N, Logue G, Rosse WF. The use of in vitro technics to study drug-induced pancytopenia. N Engl J Med. 1979 Sep 20;301(12):621–4.
Kelton, J. G., et al. “The use of in vitro technics to study drug-induced pancytopenia.N Engl J Med, vol. 301, no. 12, Sept. 1979, pp. 621–24. Pubmed, doi:10.1056/NEJM197909203011201.
Kelton JG, Huang AT, Mold N, Logue G, Rosse WF. The use of in vitro technics to study drug-induced pancytopenia. N Engl J Med. 1979 Sep 20;301(12):621–624.
Journal cover image

Published In

N Engl J Med

DOI

ISSN

0028-4793

Publication Date

September 20, 1979

Volume

301

Issue

12

Start / End Page

621 / 624

Location

United States

Related Subject Headings

  • Quinidine
  • Pancytopenia
  • Methods
  • Male
  • Leukocytes
  • Immunoglobulin G
  • Humans
  • Hematopoietic Stem Cells
  • General & Internal Medicine
  • Erythrocytes