Depth-Encoded Spectral Domain Phase Microscopy for Simultaneous Multi-Site Nanoscale Optical Measurements.


Journal Article

Spectral domain phase microscopy (SDPM) is an extension of spectral domain optical coherence tomography (SDOCT) that exploits the extraordinary phase stability of spectrometer-based systems with common-path geometry to resolve sub-wavelength displacements within a sample volume. This technique has been implemented for high resolution axial displacement and velocity measurements in biological samples, but since axial displacement information is acquired serially along the lateral dimension, it has been unable to measure fast temporal dynamics in extended samples. Depth-Encoded SDPM (DESDPM) uses multiple sample arms with unevenly spaced common path reference reflectors to multiplex independent SDPM signals from separate lateral positions on a sample simultaneously using a single interferometer, thereby reducing the time required to detect unique optical events to the integration period of the detector. Here, we introduce DESDPM and demonstrate the ability to acquire useful phase data concurrently at two laterally separated locations in a phantom sample as well as a biological preparation of spontaneously beating chick cardiomyocytes. DESDPM may be a useful tool for imaging fast cellular phenomena such as nervous conduction velocity or contractile motion.

Full Text

Duke Authors

Cited Authors

  • Hendargo, HC; Bower, BA; Reinstein, AS; Shepherd, N; Tao, YK; Izatt, JA

Published Date

  • September 2011

Published In

Volume / Issue

  • 284 / 19

Start / End Page

  • 4847 - 4851

PubMed ID

  • 21886940

Pubmed Central ID

  • 21886940

International Standard Serial Number (ISSN)

  • 0030-4018

Digital Object Identifier (DOI)

  • 10.1016/j.optcom.2011.06.001


  • eng