Investigating nanoscale cellular dynamics with cross-sectional spectral domain phase microscopy.


Journal Article

We report on cross-sectional imaging of dynamic biological specimens using a spectral domain phase microscopy (SDPM) system capable of operating at a line rate of 19 kHz. This system combines the time-sensitive capabilities of SDPM with the multi-point acquisition features of related phase-sensitive techniques. The presented phase portraits and B-scan phase images of spontaneously beating embryonic cardiomyocytes and cytoplasmic flow in A. proteus offer insight into the nature and timing of the observed cellular phenomena, demonstrating the utility of this technique for dynamic cell studies.

Full Text

Duke Authors

Cited Authors

  • Ellerbee, AK; Creazzo, TL; Izatt, JA

Published Date

  • June 2007

Published In

Volume / Issue

  • 15 / 13

Start / End Page

  • 8115 - 8124

PubMed ID

  • 19547138

Pubmed Central ID

  • 19547138

Electronic International Standard Serial Number (EISSN)

  • 1094-4087

International Standard Serial Number (ISSN)

  • 1094-4087

Digital Object Identifier (DOI)

  • 10.1364/oe.15.008115


  • eng