Spectral-domain phase microscopy.


Journal Article

Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.

Full Text

Duke Authors

Cited Authors

  • Choma, MA; Ellerbee, AK; Yang, C; Creazzo, TL; Izatt, JA

Published Date

  • May 2005

Published In

Volume / Issue

  • 30 / 10

Start / End Page

  • 1162 - 1164

PubMed ID

  • 15945141

Pubmed Central ID

  • 15945141

Electronic International Standard Serial Number (EISSN)

  • 1539-4794

International Standard Serial Number (ISSN)

  • 0146-9592

Digital Object Identifier (DOI)

  • 10.1364/ol.30.001162


  • eng