Obtaining mtDNA genomes from next-generation transcriptome sequencing: A case study on the basal Passerida (Aves: Passeriformes) phylogeny

Journal Article

Classically, the mitochondrial genome is sequenced by a series of amplicons using conserved PCR primers. Here we show how shot-gun transcriptome sequencing can be used to obtain the complete set of protein-coding genes from the mtDNA of four passerine bird species. With these sequences, we address the still unresolved basal Passerida relationships (Aves: Passeriformes). Our analysis suggests a new hypothesis for the basal relationships of Passerida, namely a clade grouping Sylvioidea and Passeroidea, with Paridae and Muscicapidae as successive sister groups to this clade. This study demonstrates the usefulness of next-generation sequencing transcriptome sequencing for obtaining new mtDNA genomes. © 2010 Elsevier Inc.

Full Text

Duke Authors

Cited Authors

  • Nabholz, B; Jarvis, ED; Ellegren, H

Published Date

  • 2010

Published In

Volume / Issue

  • 57 / 1

Start / End Page

  • 466 - 470

PubMed ID

  • 20601014

International Standard Serial Number (ISSN)

  • 1055-7903

Digital Object Identifier (DOI)

  • 10.1016/j.ympev.2010.06.009