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Obtaining mtDNA genomes from next-generation transcriptome sequencing: a case study on the basal Passerida (Aves: Passeriformes) phylogeny.

Publication ,  Journal Article
Nabholz, B; Jarvis, ED; Ellegren, H
Published in: Mol Phylogenet Evol
October 2010

Classically, the mitochondrial genome is sequenced by a series of amplicons using conserved PCR primers. Here we show how shot-gun transcriptome sequencing can be used to obtain the complete set of protein-coding genes from the mtDNA of four passerine bird species. With these sequences, we address the still unresolved basal Passerida relationships (Aves: Passeriformes). Our analysis suggests a new hypothesis for the basal relationships of Passerida, namely a clade grouping Sylvioidea and Passeroidea, with Paridae and Muscicapidae as successive sister groups to this clade. This study demonstrates the usefulness of next-generation sequencing transcriptome sequencing for obtaining new mtDNA genomes.

Duke Scholars

Published In

Mol Phylogenet Evol

DOI

EISSN

1095-9513

Publication Date

October 2010

Volume

57

Issue

1

Start / End Page

466 / 470

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Phylogeny
  • Passeriformes
  • Genome, Mitochondrial
  • Gene Expression Profiling
  • Evolutionary Biology
  • Evolution, Molecular
  • DNA, Mitochondrial
  • Animals
  • 3109 Zoology
 

Citation

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Nabholz, B., Jarvis, E. D., & Ellegren, H. (2010). Obtaining mtDNA genomes from next-generation transcriptome sequencing: a case study on the basal Passerida (Aves: Passeriformes) phylogeny. Mol Phylogenet Evol, 57(1), 466–470. https://doi.org/10.1016/j.ympev.2010.06.009
Nabholz, Benoit, Erich D. Jarvis, and Hans Ellegren. “Obtaining mtDNA genomes from next-generation transcriptome sequencing: a case study on the basal Passerida (Aves: Passeriformes) phylogeny.Mol Phylogenet Evol 57, no. 1 (October 2010): 466–70. https://doi.org/10.1016/j.ympev.2010.06.009.
Nabholz, Benoit, et al. “Obtaining mtDNA genomes from next-generation transcriptome sequencing: a case study on the basal Passerida (Aves: Passeriformes) phylogeny.Mol Phylogenet Evol, vol. 57, no. 1, Oct. 2010, pp. 466–70. Pubmed, doi:10.1016/j.ympev.2010.06.009.
Journal cover image

Published In

Mol Phylogenet Evol

DOI

EISSN

1095-9513

Publication Date

October 2010

Volume

57

Issue

1

Start / End Page

466 / 470

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Phylogeny
  • Passeriformes
  • Genome, Mitochondrial
  • Gene Expression Profiling
  • Evolutionary Biology
  • Evolution, Molecular
  • DNA, Mitochondrial
  • Animals
  • 3109 Zoology