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Ca2+/calmodulin-dependent protein kinase type IV in dorsal root ganglion: colocalization with peptides, axonal transport and effect of axotomy.

Publication ,  Journal Article
Ji, RR; Shi, TJ; Xu, ZQ; Zhang, Q; Sakagami, H; Tsubochi, H; Kondo, H; Hökfelt, T
Published in: Brain Res
May 20, 1996

Using the indirect immunofluorescence technique, the distribution of Ca2+/calmodulin-dependent protein kinase IV (CaM kinase IV) was studied in dorsal root ganglia (DRGs) and the sciatic nerve under normal circumstances and after axotomy and nerve ligation. CaM kinase IV-like immunoreactivity (-LI) was observed mainly in small DRG neurons but also in some large ones with the immunoreactivity mainly confined to the cell nuclei and with varying levels in the cytoplasm. CaM kinase IV-LI was present in around 1/4 of all CGRP-positive neurons and in the vast majority of the somatostatin-positive neurons. The enzyme levels decreased markedly after axotomy. The enzyme was also observed in axons in the sciatic nerve and accumulated both proximal and distal to a ligation. The present results suggest that CaM kinase is not of direct importance for upregulation of neuropeptides in DRG neurons after nerve injury. In addition to a nuclear function it may also play a role in the peripheral processes of DRG neurons.

Duke Scholars

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

May 20, 1996

Volume

721

Issue

1-2

Start / End Page

167 / 173

Location

Netherlands

Related Subject Headings

  • Up-Regulation
  • Sciatic Nerve
  • Rats, Sprague-Dawley
  • Rats
  • Neuropeptides
  • Neuronal Plasticity
  • Neurology & Neurosurgery
  • Male
  • Ganglia, Spinal
  • Fluorescent Antibody Technique, Indirect
 

Citation

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MLA
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Ji, R. R., Shi, T. J., Xu, Z. Q., Zhang, Q., Sakagami, H., Tsubochi, H., … Hökfelt, T. (1996). Ca2+/calmodulin-dependent protein kinase type IV in dorsal root ganglion: colocalization with peptides, axonal transport and effect of axotomy. Brain Res, 721(1–2), 167–173. https://doi.org/10.1016/0006-8993(95)01316-4
Ji, R. R., T. J. Shi, Z. Q. Xu, Q. Zhang, H. Sakagami, H. Tsubochi, H. Kondo, and T. Hökfelt. “Ca2+/calmodulin-dependent protein kinase type IV in dorsal root ganglion: colocalization with peptides, axonal transport and effect of axotomy.Brain Res 721, no. 1–2 (May 20, 1996): 167–73. https://doi.org/10.1016/0006-8993(95)01316-4.
Ji RR, Shi TJ, Xu ZQ, Zhang Q, Sakagami H, Tsubochi H, et al. Ca2+/calmodulin-dependent protein kinase type IV in dorsal root ganglion: colocalization with peptides, axonal transport and effect of axotomy. Brain Res. 1996 May 20;721(1–2):167–73.
Ji, R. R., et al. “Ca2+/calmodulin-dependent protein kinase type IV in dorsal root ganglion: colocalization with peptides, axonal transport and effect of axotomy.Brain Res, vol. 721, no. 1–2, May 1996, pp. 167–73. Pubmed, doi:10.1016/0006-8993(95)01316-4.
Ji RR, Shi TJ, Xu ZQ, Zhang Q, Sakagami H, Tsubochi H, Kondo H, Hökfelt T. Ca2+/calmodulin-dependent protein kinase type IV in dorsal root ganglion: colocalization with peptides, axonal transport and effect of axotomy. Brain Res. 1996 May 20;721(1–2):167–173.
Journal cover image

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

May 20, 1996

Volume

721

Issue

1-2

Start / End Page

167 / 173

Location

Netherlands

Related Subject Headings

  • Up-Regulation
  • Sciatic Nerve
  • Rats, Sprague-Dawley
  • Rats
  • Neuropeptides
  • Neuronal Plasticity
  • Neurology & Neurosurgery
  • Male
  • Ganglia, Spinal
  • Fluorescent Antibody Technique, Indirect