Complement component 1 inhibitor (C1-INH) like protein on murine spermatozoa: anti-C1-INH inhibits in vitro fertilization.


Journal Article

We investigated if complement component 1 inhibitor-like (C1-INH-L) protein found on human spermatozoa exists on mouse spermatozoa and is relevant to reproduction. We used Western blot analysis and immunofluorescence assays to detect C1-INH on murine sperm and tested the effects of the antibodies to C1-INH and albumin (negative control) on in vitro mouse sperm motility and fertilization. C1-INH-L, with molecular weight similar to human C1-INH (100 kDa), was present on the surface of spermatozoan head and midpiece. Treating mouse sperm with anti-C1-INH reduced the mouse sperm motility (P < 0.01), in vitro fertilization (P < 0.01) and embryo development rates (P < 0.01). Anti-albumin failed to do so. We conclude that C1-inhibitor-like protein is present on mouse sperm surface and appears to be relevant to reproduction.

Full Text

Cited Authors

  • Pillai (fka Mathur), S; Jiang, H; Roudebush, WE; Zhang, H; Waheba, M

Published Date

  • January 1998

Published In

Volume / Issue

  • 28 / 2

Start / End Page

  • 69 - 76

PubMed ID

  • 10607415

Pubmed Central ID

  • 10607415

Electronic International Standard Serial Number (EISSN)

  • 1607-842X

International Standard Serial Number (ISSN)

  • 0891-6934

Digital Object Identifier (DOI)

  • 10.3109/08916939809003869


  • eng