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Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by "helper" heterotrophic bacteria.

Publication ,  Journal Article
Morris, JJ; Kirkegaard, R; Szul, MJ; Johnson, ZI; Zinser, ER
Published in: Applied and environmental microbiology
July 2008

Axenic (pure) cultures of marine unicellular cyanobacteria of the Prochlorococcus genus grow efficiently only if the inoculation concentration is large; colonies form on semisolid medium at low efficiencies. In this work, we describe a novel method for growing Prochlorococcus colonies on semisolid agar that improves the level of recovery to approximately 100%. Prochlorococcus grows robustly at low cell concentrations, in liquid or on solid medium, when cocultured with marine heterotrophic bacteria. Once the Prochlorococcus cell concentration surpasses a critical threshold, the "helper" heterotrophs can be eliminated with antibiotics to produce axenic cultures. Our preliminary evidence suggests that one mechanism by which the heterotrophs help Prochlorococcus is the reduction of oxidative stress.

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Published In

Applied and environmental microbiology

DOI

EISSN

1098-5336

ISSN

0099-2240

Publication Date

July 2008

Volume

74

Issue

14

Start / End Page

4530 / 4534

Related Subject Headings

  • Streptomycin
  • RNA, Bacterial
  • Prochlorococcus
  • Oxidative Stress
  • Molecular Sequence Data
  • Microbiology
  • Heterotrophic Processes
  • Drug Resistance, Bacterial
  • Culture Media
  • Coculture Techniques
 

Citation

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Morris, J. J., Kirkegaard, R., Szul, M. J., Johnson, Z. I., & Zinser, E. R. (2008). Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by "helper" heterotrophic bacteria. Applied and Environmental Microbiology, 74(14), 4530–4534. https://doi.org/10.1128/aem.02479-07
Morris, J Jeffrey, Robin Kirkegaard, Martin J. Szul, Zackary I. Johnson, and Erik R. Zinser. “Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by "helper" heterotrophic bacteria.Applied and Environmental Microbiology 74, no. 14 (July 2008): 4530–34. https://doi.org/10.1128/aem.02479-07.
Morris JJ, Kirkegaard R, Szul MJ, Johnson ZI, Zinser ER. Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by "helper" heterotrophic bacteria. Applied and environmental microbiology. 2008 Jul;74(14):4530–4.
Morris, J. Jeffrey, et al. “Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by "helper" heterotrophic bacteria.Applied and Environmental Microbiology, vol. 74, no. 14, July 2008, pp. 4530–34. Epmc, doi:10.1128/aem.02479-07.
Morris JJ, Kirkegaard R, Szul MJ, Johnson ZI, Zinser ER. Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by "helper" heterotrophic bacteria. Applied and environmental microbiology. 2008 Jul;74(14):4530–4534.

Published In

Applied and environmental microbiology

DOI

EISSN

1098-5336

ISSN

0099-2240

Publication Date

July 2008

Volume

74

Issue

14

Start / End Page

4530 / 4534

Related Subject Headings

  • Streptomycin
  • RNA, Bacterial
  • Prochlorococcus
  • Oxidative Stress
  • Molecular Sequence Data
  • Microbiology
  • Heterotrophic Processes
  • Drug Resistance, Bacterial
  • Culture Media
  • Coculture Techniques