Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by "helper" heterotrophic bacteria.

Published

Journal Article

Axenic (pure) cultures of marine unicellular cyanobacteria of the Prochlorococcus genus grow efficiently only if the inoculation concentration is large; colonies form on semisolid medium at low efficiencies. In this work, we describe a novel method for growing Prochlorococcus colonies on semisolid agar that improves the level of recovery to approximately 100%. Prochlorococcus grows robustly at low cell concentrations, in liquid or on solid medium, when cocultured with marine heterotrophic bacteria. Once the Prochlorococcus cell concentration surpasses a critical threshold, the "helper" heterotrophs can be eliminated with antibiotics to produce axenic cultures. Our preliminary evidence suggests that one mechanism by which the heterotrophs help Prochlorococcus is the reduction of oxidative stress.

Full Text

Duke Authors

Cited Authors

  • Morris, JJ; Kirkegaard, R; Szul, MJ; Johnson, ZI; Zinser, ER

Published Date

  • July 2008

Published In

Volume / Issue

  • 74 / 14

Start / End Page

  • 4530 - 4534

PubMed ID

  • 18502916

Pubmed Central ID

  • 18502916

Electronic International Standard Serial Number (EISSN)

  • 1098-5336

International Standard Serial Number (ISSN)

  • 0099-2240

Digital Object Identifier (DOI)

  • 10.1128/AEM.02479-07

Language

  • eng