Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by "helper" heterotrophic bacteria.
Axenic (pure) cultures of marine unicellular cyanobacteria of the Prochlorococcus genus grow efficiently only if the inoculation concentration is large; colonies form on semisolid medium at low efficiencies. In this work, we describe a novel method for growing Prochlorococcus colonies on semisolid agar that improves the level of recovery to approximately 100%. Prochlorococcus grows robustly at low cell concentrations, in liquid or on solid medium, when cocultured with marine heterotrophic bacteria. Once the Prochlorococcus cell concentration surpasses a critical threshold, the "helper" heterotrophs can be eliminated with antibiotics to produce axenic cultures. Our preliminary evidence suggests that one mechanism by which the heterotrophs help Prochlorococcus is the reduction of oxidative stress.
Duke Scholars
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Related Subject Headings
- Streptomycin
- RNA, Bacterial
- Prochlorococcus
- Oxidative Stress
- Molecular Sequence Data
- Microbiology
- Heterotrophic Processes
- Drug Resistance, Bacterial
- Culture Media
- Coculture Techniques
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Streptomycin
- RNA, Bacterial
- Prochlorococcus
- Oxidative Stress
- Molecular Sequence Data
- Microbiology
- Heterotrophic Processes
- Drug Resistance, Bacterial
- Culture Media
- Coculture Techniques