Induction of a cellular enzyme for energy metabolism by transforming domains of adenovirus E1a.

Published

Journal Article

Brain creatine kinase is a major enzyme of cellular energy metabolism. It is overexpressed in a wide range of tumor cell lines and is used as a tumor marker. We reported recently that the promoter of the human gene has a strong sequence similarity to the adenovirus E2E promoter. This similarity suggested that the brain creatine kinase gene may be regulated by the viral activator E1a. Experiments reported here showed that both enzyme activity and mRNA levels were induced by the oncogenic products of the E1a region of adenovirus type 5, but unlike the viral E2E promoter, which is induced predominantly by E1a domain 3, brain creatine kinase induction required domains 1 and 2. These domains are important for transformation and for the association of E1a with the retinoblastoma gene product and other cellular proteins. The induction by an oncogene of a cellular gene for energy metabolism may be of significance for the metabolic events that take place after oncogenic activation.

Full Text

Duke Authors

Cited Authors

  • Kaddurah-Daouk, R; Lillie, JW; Daouk, GH; Green, MR; Kingston, R; Schimmel, P

Published Date

  • April 1990

Published In

Volume / Issue

  • 10 / 4

Start / End Page

  • 1476 - 1483

PubMed ID

  • 2138706

Pubmed Central ID

  • 2138706

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.10.4.1476

Language

  • eng

Conference Location

  • United States