Analyzing cell cycle checkpoints after ionizing radiation.

Journal Article (Journal Article)

Several methods to measure cell cycle perturbation after exposure to ionizing radiation are presented in this chapter. These methods include the G1 checkpoint assay by 5' bromode-oxyuridine (BrdUrd) labeling followed by flow cytometric analysis, the S-phase checkpoint assay by measuring DNA synthesis in response to ionizing radiation, and the G2/M checkpoint assay by quantitating histone H3 phosphorylation after ionizing radiation.

Full Text

Duke Authors

Cited Authors

  • Xu, B; Kastan, MB

Published Date

  • 2004

Published In

Volume / Issue

  • 281 /

Start / End Page

  • 283 - 292

PubMed ID

  • 15220537

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1385/1-59259-811-0:283


  • eng

Conference Location

  • United States