Interaction of FANCD2 and NBS1 in the DNA damage response.

Published

Journal Article

Fanconi anaemia (FA) and Nijmegen breakage syndrome (NBS) are autosomal recessive chromosome instability syndromes with distinct clinical phenotypes. Cells from individuals affected with FA are hypersensitive to mitomycin C (MMC), and cells from those with NBS are hypersensitive to ionizing radiation. Here we report that both NBS cell lines and individuals with NBS are hypersensitive to MMC, indicating that there may be functional linkage between FA and NBS. In wild-type cells, MMC activates the colocalization of the FA subtype D2 protein (FANCD2) and NBS1 protein in subnuclear foci. Ionizing radiation activates the ataxia telangiectasia kinase (ATM)-dependent and NBS1-dependent phosphorylation of FANCD2, resulting in an S-phase checkpoint. NBS1 and FANCD2 therefore cooperate in two distinct cellular functions, one involved in the DNA crosslink response and one involved in the S-phase checkpoint response.

Full Text

Duke Authors

Cited Authors

  • Nakanishi, K; Taniguchi, T; Ranganathan, V; New, HV; Moreau, LA; Stotsky, M; Mathew, CG; Kastan, MB; Weaver, DT; D'Andrea, AD

Published Date

  • December 2002

Published In

Volume / Issue

  • 4 / 12

Start / End Page

  • 913 - 920

PubMed ID

  • 12447395

Pubmed Central ID

  • 12447395

Electronic International Standard Serial Number (EISSN)

  • 1476-4679

International Standard Serial Number (ISSN)

  • 1465-7392

Digital Object Identifier (DOI)

  • 10.1038/ncb879

Language

  • eng