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Interaction of FANCD2 and NBS1 in the DNA damage response.

Publication ,  Journal Article
Nakanishi, K; Taniguchi, T; Ranganathan, V; New, HV; Moreau, LA; Stotsky, M; Mathew, CG; Kastan, MB; Weaver, DT; D'Andrea, AD
Published in: Nat Cell Biol
December 2002

Fanconi anaemia (FA) and Nijmegen breakage syndrome (NBS) are autosomal recessive chromosome instability syndromes with distinct clinical phenotypes. Cells from individuals affected with FA are hypersensitive to mitomycin C (MMC), and cells from those with NBS are hypersensitive to ionizing radiation. Here we report that both NBS cell lines and individuals with NBS are hypersensitive to MMC, indicating that there may be functional linkage between FA and NBS. In wild-type cells, MMC activates the colocalization of the FA subtype D2 protein (FANCD2) and NBS1 protein in subnuclear foci. Ionizing radiation activates the ataxia telangiectasia kinase (ATM)-dependent and NBS1-dependent phosphorylation of FANCD2, resulting in an S-phase checkpoint. NBS1 and FANCD2 therefore cooperate in two distinct cellular functions, one involved in the DNA crosslink response and one involved in the S-phase checkpoint response.

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Published In

Nat Cell Biol

DOI

ISSN

1465-7392

Publication Date

December 2002

Volume

4

Issue

12

Start / End Page

913 / 920

Location

England

Related Subject Headings

  • Ultraviolet Rays
  • S Phase
  • Nuclear Proteins
  • Mitomycin
  • Humans
  • Fanconi Anemia Complementation Group D2 Protein
  • Fanconi Anemia
  • Drug Resistance
  • Developmental Biology
  • DNA Repair
 

Citation

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Nakanishi, K., Taniguchi, T., Ranganathan, V., New, H. V., Moreau, L. A., Stotsky, M., … D’Andrea, A. D. (2002). Interaction of FANCD2 and NBS1 in the DNA damage response. Nat Cell Biol, 4(12), 913–920. https://doi.org/10.1038/ncb879
Nakanishi, Koji, Toshiyasu Taniguchi, Velvizhi Ranganathan, Helen V. New, Lisa A. Moreau, Maria Stotsky, Christopher G. Mathew, Michael B. Kastan, David T. Weaver, and Alan D. D’Andrea. “Interaction of FANCD2 and NBS1 in the DNA damage response.Nat Cell Biol 4, no. 12 (December 2002): 913–20. https://doi.org/10.1038/ncb879.
Nakanishi K, Taniguchi T, Ranganathan V, New HV, Moreau LA, Stotsky M, et al. Interaction of FANCD2 and NBS1 in the DNA damage response. Nat Cell Biol. 2002 Dec;4(12):913–20.
Nakanishi, Koji, et al. “Interaction of FANCD2 and NBS1 in the DNA damage response.Nat Cell Biol, vol. 4, no. 12, Dec. 2002, pp. 913–20. Pubmed, doi:10.1038/ncb879.
Nakanishi K, Taniguchi T, Ranganathan V, New HV, Moreau LA, Stotsky M, Mathew CG, Kastan MB, Weaver DT, D’Andrea AD. Interaction of FANCD2 and NBS1 in the DNA damage response. Nat Cell Biol. 2002 Dec;4(12):913–920.

Published In

Nat Cell Biol

DOI

ISSN

1465-7392

Publication Date

December 2002

Volume

4

Issue

12

Start / End Page

913 / 920

Location

England

Related Subject Headings

  • Ultraviolet Rays
  • S Phase
  • Nuclear Proteins
  • Mitomycin
  • Humans
  • Fanconi Anemia Complementation Group D2 Protein
  • Fanconi Anemia
  • Drug Resistance
  • Developmental Biology
  • DNA Repair