Skip to main content

Frequent detection of tumor cells in hematopoietic grafts in neuroblastoma and Ewing's sarcoma.

Publication ,  Journal Article
Leung, W; Chen, AR; Klann, RC; Moss, TJ; Davis, JM; Noga, SJ; Cohen, KJ; Friedman, AD; Small, D; Schwartz, CL; Borowitz, MJ; Wharam, MD ...
Published in: Bone Marrow Transplant
November 1998

Many poor-risk neuroblastomas and tumours of the Ewing's sarcoma family (ET) recur despite autologous transplants. Recurrence may be due to tumor cells contained in the BM harvests or PBSC harvests. The objectives of this prospective study were to: (1) determine the incidence and degree of tumor cell contamination in paired BM and PBSC harvests; and (2) determine the efficacy of tumor cell purging by immunomagnetic CD34+ cell selection. 198 samples from 11 consecutive patients with neuroblastoma or Ewing's sarcoma were analyzed. We assayed tumor contamination by RT-PCR assay for PGP 9.5, plus immunohistochemistry for neuroblastoma-specific antigens (the latter in neuroblastoma only). None of these patients had tumor cells detected in their BM by clinical histology immediately before BM or PBSC harvests. However, 82% of PBSC and 89% of backup BM harvests were contaminated with tumor by RT-PCR and/or immunocytochemistry assays. Unselected PBSC and BM harvests contained similar quantities of tumor cells (median, approximately 200000 cells). Cyclophosphamide plus G-CSF mobilization did not affect the incidence or level of contamination in PBSC harvests, as compared to blood obtained before mobilization. Immunomagnetic CD34+ cell selection depleted tumor cells by a median of 3.0 logs for PBSC, and 2.6 logs for BM harvests.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Bone Marrow Transplant

DOI

ISSN

0268-3369

Publication Date

November 1998

Volume

22

Issue

10

Start / End Page

971 / 979

Location

England

Related Subject Headings

  • Transplantation, Autologous
  • Survival Rate
  • Sarcoma, Ewing
  • Recurrence
  • Prospective Studies
  • Polymerase Chain Reaction
  • Neuroblastoma
  • Male
  • Immunosuppressive Agents
  • Immunomagnetic Separation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Leung, W., Chen, A. R., Klann, R. C., Moss, T. J., Davis, J. M., Noga, S. J., … Civin, C. I. (1998). Frequent detection of tumor cells in hematopoietic grafts in neuroblastoma and Ewing's sarcoma. Bone Marrow Transplant, 22(10), 971–979. https://doi.org/10.1038/sj.bmt.1701471
Leung, W., A. R. Chen, R. C. Klann, T. J. Moss, J. M. Davis, S. J. Noga, K. J. Cohen, et al. “Frequent detection of tumor cells in hematopoietic grafts in neuroblastoma and Ewing's sarcoma.Bone Marrow Transplant 22, no. 10 (November 1998): 971–79. https://doi.org/10.1038/sj.bmt.1701471.
Leung W, Chen AR, Klann RC, Moss TJ, Davis JM, Noga SJ, et al. Frequent detection of tumor cells in hematopoietic grafts in neuroblastoma and Ewing's sarcoma. Bone Marrow Transplant. 1998 Nov;22(10):971–9.
Leung, W., et al. “Frequent detection of tumor cells in hematopoietic grafts in neuroblastoma and Ewing's sarcoma.Bone Marrow Transplant, vol. 22, no. 10, Nov. 1998, pp. 971–79. Pubmed, doi:10.1038/sj.bmt.1701471.
Leung W, Chen AR, Klann RC, Moss TJ, Davis JM, Noga SJ, Cohen KJ, Friedman AD, Small D, Schwartz CL, Borowitz MJ, Wharam MD, Paidas CN, Long CA, Karandish S, McMannis JD, Kastan MB, Civin CI. Frequent detection of tumor cells in hematopoietic grafts in neuroblastoma and Ewing's sarcoma. Bone Marrow Transplant. 1998 Nov;22(10):971–979.

Published In

Bone Marrow Transplant

DOI

ISSN

0268-3369

Publication Date

November 1998

Volume

22

Issue

10

Start / End Page

971 / 979

Location

England

Related Subject Headings

  • Transplantation, Autologous
  • Survival Rate
  • Sarcoma, Ewing
  • Recurrence
  • Prospective Studies
  • Polymerase Chain Reaction
  • Neuroblastoma
  • Male
  • Immunosuppressive Agents
  • Immunomagnetic Separation