Dissociation of radiation-induced phosphorylation of replication protein A from the S-phase checkpoint.


Journal Article

Replication protein A (RPA) is a trimeric single-stranded DNA-binding protein complex involved in DNA replication, repair, and recombination. DNA damage induces phosphorylation of the RPA p34 subunit, and it has been speculated that this phosphorylation could contribute to the regulation of the DNA damage-induced S-phase checkpoint. To further examine this potential relationship, human cell lines expressing ataxia telangiectasia (AT)-mutated dominant-negative fragments, which fail to arrest in S phase in response to ionizing radiation (IR), and AT cells expressing AT-mutated-complementing fragments, which regain the ability to arrest replicative DNA synthesis in response to IR, were analyzed for radiation-induced RPA phosphorylation. Results from these studies demonstrate that IR-induced RPA phosphorylation can be uncoupled from the S-phase checkpoint, suggesting that RPA phosphorylation in response to IR is neither necessary nor sufficient for an S-phase arrest.

Full Text

Duke Authors

Cited Authors

  • Morgan, SE; Kastan, MB

Published Date

  • August 15, 1997

Published In

Volume / Issue

  • 57 / 16

Start / End Page

  • 3386 - 3389

PubMed ID

  • 9270001

Pubmed Central ID

  • 9270001

International Standard Serial Number (ISSN)

  • 0008-5472


  • eng

Conference Location

  • United States