Skip to main content

WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis.

Publication ,  Journal Article
el-Deiry, WS; Harper, JW; O'Connor, PM; Velculescu, VE; Canman, CE; Jackman, J; Pietenpol, JA; Burrell, M; Hill, DE; Wang, Y
Published in: Cancer Res
March 1, 1994

The tumor growth suppressor WAF1/CIP1 was recently shown to be induced by p53 and to be a potent inhibitor of cyclin-dependent kinases. In the present studies, we sought to determine the relationship between the expression of WAF1/CIP1 and endogenous regulation of p53 function. WAF1/CIP1 protein was first localized to the nucleus of cells containing wild-type p53 and undergoing G1 arrest. WAF1/CIP1 was induced in wild-type p53-containing cells by exposure to DNA damaging agents, but not in mutant p53-containing cells. The induction of WAF1/CIP1 protein occurred in cells undergoing either p53-associated G1 arrest or apoptosis but not in cells induced to arrest in G1 or to undergo apoptosis through p53-independent mechanisms. DNA damage led to increased levels of WAF1/CIP1 in cyclin E-containing complexes and to an associated decrease in cyclin-dependent kinase activity. These results support the idea that WAF1/CIP1 is a critical downstream effector in the p53-specific pathway of growth control in mammalian cells.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

March 1, 1994

Volume

54

Issue

5

Start / End Page

1169 / 1174

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Protein Kinases
  • Protein Kinase Inhibitors
  • Oncology & Carcinogenesis
  • Mutation
  • Mice
  • Lymphoma, T-Cell
  • Humans
  • Genes, p53
 

Citation

APA
Chicago
ICMJE
MLA
NLM
el-Deiry, W. S., Harper, J. W., O’Connor, P. M., Velculescu, V. E., Canman, C. E., Jackman, J., … Wang, Y. (1994). WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res, 54(5), 1169–1174.
Deiry, W. S. el-, J. W. Harper, P. M. O’Connor, V. E. Velculescu, C. E. Canman, J. Jackman, J. A. Pietenpol, M. Burrell, D. E. Hill, and Y. Wang. “WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis.Cancer Res 54, no. 5 (March 1, 1994): 1169–74.
el-Deiry WS, Harper JW, O’Connor PM, Velculescu VE, Canman CE, Jackman J, et al. WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994 Mar 1;54(5):1169–74.
el-Deiry, W. S., et al. “WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis.Cancer Res, vol. 54, no. 5, Mar. 1994, pp. 1169–74.
el-Deiry WS, Harper JW, O’Connor PM, Velculescu VE, Canman CE, Jackman J, Pietenpol JA, Burrell M, Hill DE, Wang Y. WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994 Mar 1;54(5):1169–1174.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

March 1, 1994

Volume

54

Issue

5

Start / End Page

1169 / 1174

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Protein Kinases
  • Protein Kinase Inhibitors
  • Oncology & Carcinogenesis
  • Mutation
  • Mice
  • Lymphoma, T-Cell
  • Humans
  • Genes, p53