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Altered cytoplasmic/nuclear distribution of the c-myc protein in differentiating ML-1 human myeloid leukemia cells.

Publication ,  Journal Article
Craig, RW; Buchan, HL; Civin, CI; Kastan, MB
Published in: Cell Growth Differ
May 1993

The c-myc gene is thought to play a role in cell proliferation and differentiation; for example, constitutive expression of an exogenously introduced c-myc gene can inhibit differentiation in hematopoietic cell lines. Expression of the endogenous c-myc gene has now been monitored during the differentiation, and associated loss of proliferation, of ML-1 human myeloblastic leukemia cells: c-myc mRNA remains detectable, at decreased levels, during differentiation along the monocyte/macrophage pathway induced with 12-O-tetradecanoylphorbol-13-acetate. c-myc protein also remains present, at undiminished levels, in mature, nonproliferative cells (assessed by immunoblotting and flow cytometry). The protein is, however, readily detectable in the cytoplasm of 12-O-tetradecanoylphorbol-13-acetate-induced cells, and some of this cytoplasmic c-myc exhibits a shift in electrophoretic mobility compared to the predominantly nuclear c-myc in uninduced cells. Furthermore, although c-myc protein continues to be synthesized in the mature cells (assessed by metabolic labeling/immunoprecipitation), loss of the protein from the cytoplasm and accumulation in the nucleus are slowed (assessed by pulse-chase metabolic labeling). These findings suggest that, during the 12-O-tetradecanoylphorbol-13-acetate-induced differentiation and loss of proliferation of ML-1 cells, c-myc protein is regulated through alterations that affect its cytoplasmic/nuclear distribution rather than its total cellular content.

Duke Scholars

Published In

Cell Growth Differ

ISSN

1044-9523

Publication Date

May 1993

Volume

4

Issue

5

Start / End Page

349 / 357

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Tetradecanoylphorbol Acetate
  • Proto-Oncogene Proteins c-myc
  • Oncology & Carcinogenesis
  • Nuclear Proteins
  • Leukemia, Myeloid
  • Humans
  • Developmental Biology
  • Cytoplasm
  • Cell Nucleus
 

Citation

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Craig, R. W., Buchan, H. L., Civin, C. I., & Kastan, M. B. (1993). Altered cytoplasmic/nuclear distribution of the c-myc protein in differentiating ML-1 human myeloid leukemia cells. Cell Growth Differ, 4(5), 349–357.
Craig, R. W., H. L. Buchan, C. I. Civin, and M. B. Kastan. “Altered cytoplasmic/nuclear distribution of the c-myc protein in differentiating ML-1 human myeloid leukemia cells.Cell Growth Differ 4, no. 5 (May 1993): 349–57.
Craig RW, Buchan HL, Civin CI, Kastan MB. Altered cytoplasmic/nuclear distribution of the c-myc protein in differentiating ML-1 human myeloid leukemia cells. Cell Growth Differ. 1993 May;4(5):349–57.
Craig, R. W., et al. “Altered cytoplasmic/nuclear distribution of the c-myc protein in differentiating ML-1 human myeloid leukemia cells.Cell Growth Differ, vol. 4, no. 5, May 1993, pp. 349–57.
Craig RW, Buchan HL, Civin CI, Kastan MB. Altered cytoplasmic/nuclear distribution of the c-myc protein in differentiating ML-1 human myeloid leukemia cells. Cell Growth Differ. 1993 May;4(5):349–357.

Published In

Cell Growth Differ

ISSN

1044-9523

Publication Date

May 1993

Volume

4

Issue

5

Start / End Page

349 / 357

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Tetradecanoylphorbol Acetate
  • Proto-Oncogene Proteins c-myc
  • Oncology & Carcinogenesis
  • Nuclear Proteins
  • Leukemia, Myeloid
  • Humans
  • Developmental Biology
  • Cytoplasm
  • Cell Nucleus