Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.

Published

Journal Article

The primary cilium is an antenna-like organelle that is dynamically regulated during the cell cycle. Ciliogenesis is initiated as cells enter quiescence, whereas resorption of the cilium precedes mitosis. The mechanisms coordinating ciliogenesis with the cell cycle are unknown. Here we identify the centrosomal protein Nde1 (nuclear distribution gene E homologue 1) as a negative regulator of ciliary length. Nde1 is expressed at high levels in mitosis, low levels in quiescence and localizes at the mother centriole, which nucleates the primary cilium. Cells depleted of Nde1 have longer cilia and a delay in cell cycle re-entry that correlates with ciliary length. Knockdown of Nde1 in zebrafish embryos results in increased ciliary length, suppression of cell division, reduction of the number of cells forming the Kupffer's vesicle and left-right patterning defects. These data suggest that Nde1 is an integral component of a network coordinating ciliary length with cell cycle progression and have implications for understanding the transition from a quiescent to a proliferative state.

Full Text

Duke Authors

Cited Authors

  • Kim, S; Zaghloul, NA; Bubenshchikova, E; Oh, EC; Rankin, S; Katsanis, N; Obara, T; Tsiokas, L

Published Date

  • April 2011

Published In

Volume / Issue

  • 13 / 4

Start / End Page

  • 351 - 360

PubMed ID

  • 21394081

Pubmed Central ID

  • 21394081

Electronic International Standard Serial Number (EISSN)

  • 1476-4679

Digital Object Identifier (DOI)

  • 10.1038/ncb2183

Language

  • eng

Conference Location

  • England