Disruption of Bardet-Biedl syndrome ciliary proteins perturbs planar cell polarity in vertebrates.


Journal Article

The evolutionarily conserved planar cell polarity (PCP) pathway (or noncanonical Wnt pathway) drives several important cellular processes, including epithelial cell polarization, cell migration and mitotic spindle orientation. In vertebrates, PCP genes have a vital role in polarized convergent extension movements during gastrulation and neurulation. Here we show that mice with mutations in genes involved in Bardet-Biedl syndrome (BBS), a disorder associated with ciliary dysfunction, share phenotypes with PCP mutants including open eyelids, neural tube defects and disrupted cochlear stereociliary bundles. Furthermore, we identify genetic interactions between BBS genes and a PCP gene in both mouse (Ltap, also called Vangl2) and zebrafish (vangl2). In zebrafish, the augmented phenotype results from enhanced defective convergent extension movements. We also show that Vangl2 localizes to the basal body and axoneme of ciliated cells, a pattern reminiscent of that of the BBS proteins. These data suggest that cilia are intrinsically involved in PCP processes.

Full Text

Duke Authors

Cited Authors

  • Ross, AJ; May-Simera, H; Eichers, ER; Kai, M; Hill, J; Jagger, DJ; Leitch, CC; Chapple, JP; Munro, PM; Fisher, S; Tan, PL; Phillips, HM; Leroux, MR; Henderson, DJ; Murdoch, JN; Copp, AJ; Eliot, M-M; Lupski, JR; Kemp, DT; Dollfus, H; Tada, M; Katsanis, N; Forge, A; Beales, PL

Published Date

  • October 2005

Published In

Volume / Issue

  • 37 / 10

Start / End Page

  • 1135 - 1140

PubMed ID

  • 16170314

Pubmed Central ID

  • 16170314

International Standard Serial Number (ISSN)

  • 1061-4036

Digital Object Identifier (DOI)

  • 10.1038/ng1644


  • eng

Conference Location

  • United States