Skip to main content

In vivo optical imaging of human vaginal gel thickness distributions with a probe-based, dual-modality instrument.

Publication ,  Journal Article
Drake, TK; DeSoto, MG; Peters, JJ; Henderson, MH; Thiele, B; Bishop, TS; Murtha, AP; Katz, DF; Wax, A
Published in: Journal of biomedical optics
November 2012

ABSTRACT. We used a probe-based dual-modality optical imaging instrument to measure in vivo coating thickness distributions of a gel distributed along the vaginal lumen, in a clinical study. The gel was a surrogate for one delivering an anti-HIV topical microbicide. Imaging data from Fourier-domain multiplexed low-coherence interferometry (mLCI) and fluorimetric measurements were compared to assess the feasibility and accuracy of mLCI in measuring in vivo gel coating thickness distributions. In each study session, 3.5 mL of Replens gel was inserted to the vaginal fornix while the participant was supine. The participant either: 1. remained supine (10 or 60 min); or 2. sat up (1 min), stood up (1 min), sat down (1 min) and returned to the supine position; net elapsed time was 10 or 60 min after which the gel distribution was imaged. Local coating thickness distributions were qualitatively and quantitatively similar. Here mLCI did not accurately measure thicker gel coatings (>0.8  mm), a limitation not seen with fluorimetry. However, mLCI is capable of measuring in vivo microbicide gel distributions with resolution on the order of 10 μm, without the need for exogenous contrast agents, and can accurately capture relevant summary coating measures in good agreement with fluorimetry.

Duke Scholars

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

November 2012

Volume

17

Issue

11

Start / End Page

116014

Related Subject Headings

  • Young Adult
  • Vaginal Creams, Foams, and Jellies
  • Supine Position
  • Optics
  • Optical Phenomena
  • Middle Aged
  • Lipids
  • Interferometry
  • Humans
  • Gels
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Drake, T. K., DeSoto, M. G., Peters, J. J., Henderson, M. H., Thiele, B., Bishop, T. S., … Wax, A. (2012). In vivo optical imaging of human vaginal gel thickness distributions with a probe-based, dual-modality instrument. Journal of Biomedical Optics, 17(11), 116014. https://doi.org/10.1117/1.jbo.17.11.116014
Drake, Tyler K., Michael G. DeSoto, Jennifer J. Peters, Marcus H. Henderson, Bonnie Thiele, Tammy Sinclair Bishop, Amy P. Murtha, David F. Katz, and Adam Wax. “In vivo optical imaging of human vaginal gel thickness distributions with a probe-based, dual-modality instrument.Journal of Biomedical Optics 17, no. 11 (November 2012): 116014. https://doi.org/10.1117/1.jbo.17.11.116014.
Drake TK, DeSoto MG, Peters JJ, Henderson MH, Thiele B, Bishop TS, et al. In vivo optical imaging of human vaginal gel thickness distributions with a probe-based, dual-modality instrument. Journal of biomedical optics. 2012 Nov;17(11):116014.
Drake, Tyler K., et al. “In vivo optical imaging of human vaginal gel thickness distributions with a probe-based, dual-modality instrument.Journal of Biomedical Optics, vol. 17, no. 11, Nov. 2012, p. 116014. Epmc, doi:10.1117/1.jbo.17.11.116014.
Drake TK, DeSoto MG, Peters JJ, Henderson MH, Thiele B, Bishop TS, Murtha AP, Katz DF, Wax A. In vivo optical imaging of human vaginal gel thickness distributions with a probe-based, dual-modality instrument. Journal of biomedical optics. 2012 Nov;17(11):116014.

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

November 2012

Volume

17

Issue

11

Start / End Page

116014

Related Subject Headings

  • Young Adult
  • Vaginal Creams, Foams, and Jellies
  • Supine Position
  • Optics
  • Optical Phenomena
  • Middle Aged
  • Lipids
  • Interferometry
  • Humans
  • Gels