The evolution of hamster sperm motility during capacitation and interaction with the ovum vestments in vitro

Journal Article (Journal Article)

Movement characteristics of golden hamster spermatozoa were studied upon collection from the cauda epididymis, during an incubation which capacitates the spermatozoa in vitro, during penetration of the cumulus, and during attachment to and penetration of the zona pellucida. High‐speed videomicrography was employed to quantitate flagellar beat frequency and shape. The status of the acrosome was also assessed. During capacitation, hamster spermatozoa become increasingly invigorated before the onset of hyperactivated motility. Within the cumulus, beat frequency and curvature are reduced, apparently in response to the physical resistive properties of the matrix material. These properties appear to vary within the cumulus. Initial attachment to the zona precedes completion of the acrosome reaction, is non‐rigid, and is accompanied by increased beat frequency and curvature. Subsequently, the onset of rigid binding to the zona, completion of the acrosome reaction, and increased flagellar beat frequency are very closely associated in time. The latter produces an increase in thrust against the zona. Preliminary results indicate that ensuing zona penetration requires not more than five minutes, is at oblique angles, and is associated with a continuation of vigorous flagellar beating. Copyright © 1986 Alan R. Liss, Inc.

Full Text

Duke Authors

Cited Authors

  • Katz, DF; Cherr, GN; Lambert, H

Published Date

  • January 1, 1986

Published In

Volume / Issue

  • 14 / 4

Start / End Page

  • 333 - 346

Electronic International Standard Serial Number (EISSN)

  • 1554-3919

International Standard Serial Number (ISSN)

  • 0148-7280

Digital Object Identifier (DOI)

  • 10.1002/mrd.1120140406

Citation Source

  • Scopus