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Activity-dependent expression of RNA binding protein HuD and its association with mRNAs in neurons.

Publication ,  Journal Article
Tiruchinapalli, DM; Ehlers, MD; Keene, JD
Published in: RNA Biol
2008

The dendritic trafficking of RNA binding proteins (RBPs) is an important posttranscriptional process involved in the regulation of synaptic plasticity. For example, HuD RBP binds to AU-rich elements (AREs) in the 3' untranslated regions (3'UTR) of immediate-early gene (IEG) transcripts, whose protein products directly affect synaptic plasticity. However, the subcellular localization of HuD RBPs and associated mRNAs has not been investigated following neuronal stimulation. Immunofluorescence analysis revealed activity-dependent dendritic localization of HuD RBPs following KCl stimulation in hippocampal neurons, while immunoprecipitation demonstrated the association of HuD RBP with neuronal mRNAs encoding neuritin, Homer1a, GAP-43, Neuroligins, Verge and CAMKIIalpha. Activity-dependent expression of HuD involves activation of NMDAR as NMDA receptor 1 knockout mice (Nr1(neo-/-)) exhibited decreased expression of HuD. Moreover, translational regulation of HuD-associated transcripts was suggested by its co-localization with poly-A-binding protein (PABP) as well as the cap-binding protein (eIF4E). We propose that post-transcriptional regulation of neuronal mRNAs by HuD RBPs mediates protein synthesis-dependent changes in synaptic plasticity.

Duke Scholars

Published In

RNA Biol

DOI

EISSN

1555-8584

Publication Date

2008

Volume

5

Issue

3

Start / End Page

157 / 168

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Subcellular Fractions
  • Rats
  • RNA, Messenger
  • Protein Transport
  • Potassium Chloride
  • Polyribosomes
  • Neurons
  • Mice
  • Membrane Potentials
 

Citation

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Tiruchinapalli, D. M., Ehlers, M. D., & Keene, J. D. (2008). Activity-dependent expression of RNA binding protein HuD and its association with mRNAs in neurons. RNA Biol, 5(3), 157–168. https://doi.org/10.4161/rna.5.3.6782
Tiruchinapalli, Dhanrajan M., Michael D. Ehlers, and Jack D. Keene. “Activity-dependent expression of RNA binding protein HuD and its association with mRNAs in neurons.RNA Biol 5, no. 3 (2008): 157–68. https://doi.org/10.4161/rna.5.3.6782.
Tiruchinapalli DM, Ehlers MD, Keene JD. Activity-dependent expression of RNA binding protein HuD and its association with mRNAs in neurons. RNA Biol. 2008;5(3):157–68.
Tiruchinapalli, Dhanrajan M., et al. “Activity-dependent expression of RNA binding protein HuD and its association with mRNAs in neurons.RNA Biol, vol. 5, no. 3, 2008, pp. 157–68. Pubmed, doi:10.4161/rna.5.3.6782.
Tiruchinapalli DM, Ehlers MD, Keene JD. Activity-dependent expression of RNA binding protein HuD and its association with mRNAs in neurons. RNA Biol. 2008;5(3):157–168.

Published In

RNA Biol

DOI

EISSN

1555-8584

Publication Date

2008

Volume

5

Issue

3

Start / End Page

157 / 168

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Subcellular Fractions
  • Rats
  • RNA, Messenger
  • Protein Transport
  • Potassium Chloride
  • Polyribosomes
  • Neurons
  • Mice
  • Membrane Potentials