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Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.

Publication ,  Journal Article
Gao, FB; Carson, CC; Levine, T; Keene, JD
Published in: Proc Natl Acad Sci U S A
November 8, 1994

Hel-N1, a human RNA-binding protein, shares significant homology with Drosophila protein ELAV, which is essential for fly neuronal development. Hel-N1 has been shown to bind in vitro to 3' untranslated regions of mRNAs encoding c-myc, c-fos, granulocyte/macrophage colony-stimulating factor, and transcriptional repressor, Id. We report that Hel-N1 and a related form, Hel-N2, are expressed in human medulloblastoma cells, but their ratio differs significantly from that in adult brain and fetal brain. Selection of RNA targets from randomized combinatorial libraries yielded (A+U)-rich consensus sequences for both Hel-N1 and Hel-N2. As a means to identify cellular RNA targets for these proteins, we devised combinatorial shape libraries representing naturally derived 3' untranslated regions and were able to select a structurally related subset of transcripts that bound to Hel-N1. Approximately 10% of the proteins encoded by these subset mRNAs were identifiable in the data bases and most are implicated in cell growth regulation. This approach provides a means to gain access to novel genes expressed in various cell types by partitioning mRNAs containing common sequence elements using RNA-binding proteins.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 8, 1994

Volume

91

Issue

23

Start / End Page

11207 / 11211

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Sequence Homology, Nucleic Acid
  • Sequence Alignment
  • RNA-Binding Proteins
  • RNA, Messenger
  • Nerve Tissue Proteins
  • Molecular Sequence Data
  • Ligands
  • In Vitro Techniques
  • Humans
 

Citation

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Gao, F. B., Carson, C. C., Levine, T., & Keene, J. D. (1994). Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A, 91(23), 11207–11211. https://doi.org/10.1073/pnas.91.23.11207
Gao, F. B., C. C. Carson, T. Levine, and J. D. Keene. “Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.Proc Natl Acad Sci U S A 91, no. 23 (November 8, 1994): 11207–11. https://doi.org/10.1073/pnas.91.23.11207.
Gao FB, Carson CC, Levine T, Keene JD. Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11207–11.
Gao, F. B., et al. “Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.Proc Natl Acad Sci U S A, vol. 91, no. 23, Nov. 1994, pp. 11207–11. Pubmed, doi:10.1073/pnas.91.23.11207.
Gao FB, Carson CC, Levine T, Keene JD. Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11207–11211.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 8, 1994

Volume

91

Issue

23

Start / End Page

11207 / 11211

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Sequence Homology, Nucleic Acid
  • Sequence Alignment
  • RNA-Binding Proteins
  • RNA, Messenger
  • Nerve Tissue Proteins
  • Molecular Sequence Data
  • Ligands
  • In Vitro Techniques
  • Humans