Polycistronic vesicular stomatitis virus RNA transcripts.
Journal Article (Journal Article)
A procedure to enrich for the sequences present at the junction between the linked messages in the polycistronic RNAs symthesized in vitro by vesicular stomatitis virus (VSV) is described. Analyses of these sequences show that they contain a precise transcript of both the intercistronic dinucleotide and the pentanucleotide 5'--C-U-G-U-U--3', common to the 5'-end of all VSV cistrons, covalently linked to the 3'-side of the intervening poly(A). The data strongly suggest that the VSV transcriptase polyadenylylates the mRNAs and can then resume direct and precise transcription of the genome-without reinitiation and without skipping nucleotides.
Full Text
Duke Authors
Cited Authors
- Herman, RC; Schubert, M; Keene, JD; Lazzarini, RA
Published Date
- August 1, 1980
Published In
Volume / Issue
- 77 / 8
Start / End Page
- 4662 - 4665
PubMed ID
- 6254036
Pubmed Central ID
- PMC349905
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.77.8.4662
Language
- eng
Conference Location
- United States