Polycistronic vesicular stomatitis virus RNA transcripts.

Journal Article (Journal Article)

A procedure to enrich for the sequences present at the junction between the linked messages in the polycistronic RNAs symthesized in vitro by vesicular stomatitis virus (VSV) is described. Analyses of these sequences show that they contain a precise transcript of both the intercistronic dinucleotide and the pentanucleotide 5'--C-U-G-U-U--3', common to the 5'-end of all VSV cistrons, covalently linked to the 3'-side of the intervening poly(A). The data strongly suggest that the VSV transcriptase polyadenylylates the mRNAs and can then resume direct and precise transcription of the genome-without reinitiation and without skipping nucleotides.

Full Text

Duke Authors

Cited Authors

  • Herman, RC; Schubert, M; Keene, JD; Lazzarini, RA

Published Date

  • August 1, 1980

Published In

Volume / Issue

  • 77 / 8

Start / End Page

  • 4662 - 4665

PubMed ID

  • 6254036

Pubmed Central ID

  • PMC349905

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.77.8.4662


  • eng

Conference Location

  • United States