Site on the vesicular stomatitis virus genome specifying polyadenylation and the end of the L gene mRNA.

Published

Journal Article

The 5'-terminal nucleotide sequence from positions 50 to 130 of vesicular stomatitis virus RNA was determined indirectly by using a defective interfering particle RNA which contains covalently linked genomic minus and antigenomic plus sense RNAs. The last 18 nucleotides of the L gene coding for in the viral polymerase were identified and isolated by specific duplex formation between 5' terminally labeled oligonucleotides from a small single-stranded defective interfering particle RNA and L gene mRNA. The L gene ends at position 60 from the 5' terminus of the vesicular stomatitis genome. The data demonstrated that the first seven adenine residues in the polyadenylic acid tail of L gene mRNA may be coded for in the genome and suggested that the viral transcriptase itself may carry out polyadenylation, possibly by chattering at the uridine-rich sequence at the end of the L gene. Analysis of the 5'-terminal sequence of vesicular stomatitis virus genomic RNA revealed that it might fold into a complex secondary structure with possibly 62% of the bases paired.

Full Text

Duke Authors

Cited Authors

  • Schubert, M; Keene, JD; Herman, RC; Lazzarini, RA

Published Date

  • May 1, 1980

Published In

Volume / Issue

  • 34 / 2

Start / End Page

  • 550 - 559

PubMed ID

  • 6246280

Pubmed Central ID

  • 6246280

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States