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Intervening sequence between the leader region and the nucleopcapsid gene of vesicular stomatitis virus RNA.

Publication ,  Journal Article
Keene, JD; Schubert, M; Lazzarini, RA
Published in: J Virol
February 1980

The base sequence at the 3' end of vesicular stomatitis virus RNA was determined by using terminal labels and chemical RNA sequencing. The leader RNA was complementary to 47 bases at the 3' terminus, whereas the nucleocapsid gene (N) began 51 nucleotides from the 3' end of the genomic RNA. The intervening bases were 3'...GAAA...5' for the Indiana serotype and 3'...GAAAA...5' for the New Jersey serotype. The complements of these bases did not appear in either the leader RNA or the N mRNA. This sequence may function as a stop signal or cleavage site during transcription. Furthermore, processing or termination at this sequence must be inhibited during the production of full-length RNA plus-sense strands (replication). We recently found similar sequences approximately 46 to 48 nucleotides from the 3' ends of several defective interfering particle RNAs where the short defective interfering particle transciption products terminate. This sequence is present also at the end of the polymerase (L) gene.

Duke Scholars

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

February 1980

Volume

33

Issue

2

Start / End Page

789 / 794

Location

United States

Related Subject Headings

  • Virology
  • Vesiculovirus
  • Vesicular stomatitis Indiana virus
  • RNA, Viral
  • Poly A
  • Genes, Viral
  • Gene Expression Regulation
  • Capsid
  • Base Sequence
  • 32 Biomedical and clinical sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Keene, J. D., Schubert, M., & Lazzarini, R. A. (1980). Intervening sequence between the leader region and the nucleopcapsid gene of vesicular stomatitis virus RNA. J Virol, 33(2), 789–794. https://doi.org/10.1128/JVI.33.2.789-794.1980
Keene, J. D., M. Schubert, and R. A. Lazzarini. “Intervening sequence between the leader region and the nucleopcapsid gene of vesicular stomatitis virus RNA.J Virol 33, no. 2 (February 1980): 789–94. https://doi.org/10.1128/JVI.33.2.789-794.1980.
Keene, J. D., et al. “Intervening sequence between the leader region and the nucleopcapsid gene of vesicular stomatitis virus RNA.J Virol, vol. 33, no. 2, Feb. 1980, pp. 789–94. Pubmed, doi:10.1128/JVI.33.2.789-794.1980.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

February 1980

Volume

33

Issue

2

Start / End Page

789 / 794

Location

United States

Related Subject Headings

  • Virology
  • Vesiculovirus
  • Vesicular stomatitis Indiana virus
  • RNA, Viral
  • Poly A
  • Genes, Viral
  • Gene Expression Regulation
  • Capsid
  • Base Sequence
  • 32 Biomedical and clinical sciences