Nucleotide sequence homology at the 3' termini of RNA from vesicular stomatitis virus and its defective interfering particles.

Published

Journal Article

Vesicular stomatitis virus (VSV) and defective interfering (DI) particle RNAs were labeled at their 3' ends by using RNA ligase and cytidine 3',5'-bis[32P]phosphate. The RNAs were subjected to partial digestion with alkali and analyzed by oligonucleotide fingerprinting in two dimensions. VSV and DI particle RNAs have complete sequence homology for the first eight bases from the 3' end. The following four positions contain three mismatched nucleotides in which guanosine residues in one strand are replaced by uridine residues in the other. There is again complete homology for the next five bases (positions 13-17). The locations of purine residues within the sequence were confirmed by partial digestion with RNase T1 and RNase U2 and separation by size on 20% acrylamide gels. The latter method also indicated that sequences of VSV and DI particle RNAs diverge beyond the 18th nucleotide from the 3' termini.

Full Text

Duke Authors

Cited Authors

  • Keene, JD; Schubert, M; Lazzarini, RA; Rosenberg, M

Published Date

  • July 1, 1978

Published In

Volume / Issue

  • 75 / 7

Start / End Page

  • 3225 - 3229

PubMed ID

  • 210454

Pubmed Central ID

  • 210454

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.75.7.3225

Language

  • eng

Conference Location

  • United States