Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I.

Published

Journal Article

The ribonuclease (RNase) H class of enzymes degrades the RNA component of RNA:DNA hybrids and is important in nucleic acid metabolism. RNase H2 is specialized to remove single ribonucleotides [ribonucleoside monophosphates (rNMPs)] from duplex DNA, and its absence in budding yeast has been associated with the accumulation of deletions within short tandem repeats. Here, we demonstrate that rNMP-associated deletion formation requires the activity of Top1, a topoisomerase that relaxes supercoils by reversibly nicking duplex DNA. The reported studies extend the role of Top1 to include the processing of rNMPs in genomic DNA into irreversible single-strand breaks, an activity that can have distinct mutagenic consequences and may be relevant to human disease.

Full Text

Duke Authors

Cited Authors

  • Kim, N; Huang, S-YN; Williams, JS; Li, YC; Clark, AB; Cho, J-E; Kunkel, TA; Pommier, Y; Jinks-Robertson, S

Published Date

  • June 24, 2011

Published In

Volume / Issue

  • 332 / 6037

Start / End Page

  • 1561 - 1564

PubMed ID

  • 21700875

Pubmed Central ID

  • 21700875

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Digital Object Identifier (DOI)

  • 10.1126/science.1205016

Language

  • eng

Conference Location

  • United States