Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I.
Journal Article (Journal Article)
The ribonuclease (RNase) H class of enzymes degrades the RNA component of RNA:DNA hybrids and is important in nucleic acid metabolism. RNase H2 is specialized to remove single ribonucleotides [ribonucleoside monophosphates (rNMPs)] from duplex DNA, and its absence in budding yeast has been associated with the accumulation of deletions within short tandem repeats. Here, we demonstrate that rNMP-associated deletion formation requires the activity of Top1, a topoisomerase that relaxes supercoils by reversibly nicking duplex DNA. The reported studies extend the role of Top1 to include the processing of rNMPs in genomic DNA into irreversible single-strand breaks, an activity that can have distinct mutagenic consequences and may be relevant to human disease.
- Kim, N; Huang, S-YN; Williams, JS; Li, YC; Clark, AB; Cho, J-E; Kunkel, TA; Pommier, Y; Jinks-Robertson, S
- June 24, 2011
Volume / Issue
- 332 / 6037
Start / End Page
- 1561 - 1564
Pubmed Central ID
Electronic International Standard Serial Number (EISSN)
Digital Object Identifier (DOI)
- United States