Cordon-bleu is an actin nucleation factor and controls neuronal morphology.

Published

Journal Article

Despite the wealth of different actin structures formed, only two actin nucleation factors are well established in vertebrates: the Arp2/3 complex and formins. Here, we describe a further nucleator, cordon-bleu (Cobl). Cobl is a brain-enriched protein using three Wiskott-Aldrich syndrome protein homology 2 (WH2) domains for actin binding. Cobl promotes nonbundled, unbranched filaments. Filament formation relies on barbed-end growth and requires all three Cobl WH2 domains and the extended linker L2. We suggest that the nucleation power of Cobl is based on the assembly of three actin monomers in cross-filament orientation. Cobl localizes to sites of high actin dynamics and modulates cell morphology. In neurons, induction of both neurites and neurite branching is dramatically increased by Cobl expression-effects that critically depend on Cobl's actin nucleation ability. Correspondingly, Cobl depletion results in decreased dendritic arborization. Thus, Cobl is an actin nucleator controlling neuronal morphology and development.

Full Text

Duke Authors

Cited Authors

  • Ahuja, R; Pinyol, R; Reichenbach, N; Custer, L; Klingensmith, J; Kessels, MM; Qualmann, B

Published Date

  • October 19, 2007

Published In

Volume / Issue

  • 131 / 2

Start / End Page

  • 337 - 350

PubMed ID

  • 17956734

Pubmed Central ID

  • 17956734

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2007.08.030

Language

  • eng

Conference Location

  • United States