Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines.

Published online

Journal Article

BACKGROUND: Integrase defective lentiviral vectors (IDLV) represent a promising delivery system for immunization purposes. Human dendritic cells (DC) are the main cell types mediating the immune response and are readily transduced by IDLV, allowing effective triggering of in vitro expansion of antigen-specific primed CD8+ T cells. However, IDLV expression in transduced DC is at lower levels than those of the integrase (IN) competent counterpart, thus requiring further improvement of IDLV for future use in the clinic. RESULTS: In this paper we show that the addition of simian immunodeficiency (SIV)-Vpx protein in the vector preparation greatly improves transduction of human and simian DC, but not of murine DC, thus increasing the ability of transduced DC to act as functional antigen presenting cells, in the absence of integrated vector sequences. Importantly, the presence of SIV-Vpx allows for using lower dose of input IDLV during in vitro transduction, thus further improving the IDLV safety profile. CONCLUSIONS: These results have significant implications for the development of IDLV-based vaccines.

Full Text

Duke Authors

Cited Authors

  • Negri, DRM; Rossi, A; Blasi, M; Michelini, Z; Leone, P; Chiantore, MV; Baroncelli, S; Perretta, G; Cimarelli, A; Klotman, ME; Cara, A

Published Date

  • August 22, 2012

Published In

Volume / Issue

  • 9 /

Start / End Page

  • 69 -

PubMed ID

  • 22913641

Pubmed Central ID

  • 22913641

Electronic International Standard Serial Number (EISSN)

  • 1742-4690

Digital Object Identifier (DOI)

  • 10.1186/1742-4690-9-69

Language

  • eng

Conference Location

  • England