Virological synapses allow HIV-1 uptake and gene expression in renal tubular epithelial cells.

Published

Journal Article

In animal models of HIV-associated nephropathy, the expression of HIV regulatory genes in epithelial cells is sufficient to cause disease, but how the CD4-negative epithelial cells come to express HIV genes is unknown. Here, we co-cultured T cells infected with fluorescently tagged HIV with renal tubular epithelial cells and observed efficient virus transfer between these cells. The quantity of HIV transferred was much greater than that achieved by exposure to large amounts of cell-free virus and occurred without a requirement for CD4 or Env. The transfer required stable cell-cell adhesion, which could be blocked by sulfated polysaccharides or poly-anionic compounds. We found that the internalization of virus could lead to de novo synthesis of viral protein from incoming viral RNAs even in the presence of a reverse transcriptase inhibitor. These results illustrate an interaction between infected T cells and nonimmune cells, supporting the presence of virological synapses between HIV-harboring T cells and renal tubular epithelial cells, allowing viral uptake and gene expression in epithelial cells.

Full Text

Duke Authors

Cited Authors

  • Chen, P; Chen, BK; Mosoian, A; Hays, T; Ross, MJ; Klotman, PE; Klotman, ME

Published Date

  • March 2011

Published In

Volume / Issue

  • 22 / 3

Start / End Page

  • 496 - 507

PubMed ID

  • 21335514

Pubmed Central ID

  • 21335514

Electronic International Standard Serial Number (EISSN)

  • 1533-3450

International Standard Serial Number (ISSN)

  • 1046-6673

Digital Object Identifier (DOI)

  • 10.1681/ASN.2010040379

Language

  • eng