Skip to main content
Journal cover image

HIV-1 Vpr inhibits cytokinesis in human proximal tubule cells.

Publication ,  Journal Article
Rosenstiel, PE; Gruosso, T; Letourneau, AM; Chan, JJ; LeBlanc, A; Husain, M; Najfeld, V; Planelles, V; D'Agati, VD; Klotman, ME; Klotman, PE
Published in: Kidney Int
October 2008

Transgenic mouse models of HIV-associated nephropathy (HIVAN) show that expression of HIV-1 genes in kidney cells produces collapsing focal segmental glomerulosclerosis and microcystic tubular disease typical of the human disease. HIV-1 vpr plays an important role in the glomerulosclerosis of HIVAN, especially when it is associated with nef expression in podocytes. Further, Vpr is reported to exacerbate tubular pathology. Here we determined effects of vpr expression on renal tubular epithelial cell function by transducing them with a pseudotyped lentivirus vector carrying HIV-1 vpr and control genes. Vpr expression in the cultured cells impaired cytokinesis causing cell enlargement and multinucleation. This profound in vitro phenotype caused us to reexamine the HIVAN mouse model and human HIVAN biopsies to see if similar changes occur in vivo. Both showed abundant hypertrophic tubule cells similar to the in vitro finding that represents a previously unappreciated aspect of the human disease. Additionally, multinucleated tubular cells were identified in the murine HIVAN model and increased chromosome number was detected in tubular cells of human HIVAN biopsies. Our study provides evidence of a new clinical phenotype in HIVAN that may result from the ability of Vpr to impair cytokinesis.

Duke Scholars

Published In

Kidney Int

DOI

EISSN

1523-1755

Publication Date

October 2008

Volume

74

Issue

8

Start / End Page

1049 / 1058

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Transduction, Genetic
  • Mice
  • Kidney Tubules, Proximal
  • Humans
  • Human Immunodeficiency Virus Proteins
  • HIV-1
  • Giant Cells
  • Gene Products, vpr
  • Epithelial Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Rosenstiel, P. E., Gruosso, T., Letourneau, A. M., Chan, J. J., LeBlanc, A., Husain, M., … Klotman, P. E. (2008). HIV-1 Vpr inhibits cytokinesis in human proximal tubule cells. Kidney International, 74(8), 1049–1058. https://doi.org/10.1038/ki.2008.303
Rosenstiel, Paul E., Tina Gruosso, Audrey M. Letourneau, Justin J. Chan, Amanda LeBlanc, Mohammad Husain, Vesna Najfeld, et al. “HIV-1 Vpr inhibits cytokinesis in human proximal tubule cells.Kidney International 74, no. 8 (October 2008): 1049–58. https://doi.org/10.1038/ki.2008.303.
Rosenstiel PE, Gruosso T, Letourneau AM, Chan JJ, LeBlanc A, Husain M, et al. HIV-1 Vpr inhibits cytokinesis in human proximal tubule cells. Kidney international. 2008 Oct;74(8):1049–58.
Rosenstiel, Paul E., et al. “HIV-1 Vpr inhibits cytokinesis in human proximal tubule cells.Kidney International, vol. 74, no. 8, Oct. 2008, pp. 1049–58. Epmc, doi:10.1038/ki.2008.303.
Rosenstiel PE, Gruosso T, Letourneau AM, Chan JJ, LeBlanc A, Husain M, Najfeld V, Planelles V, D’Agati VD, Klotman ME, Klotman PE. HIV-1 Vpr inhibits cytokinesis in human proximal tubule cells. Kidney international. 2008 Oct;74(8):1049–1058.
Journal cover image

Published In

Kidney Int

DOI

EISSN

1523-1755

Publication Date

October 2008

Volume

74

Issue

8

Start / End Page

1049 / 1058

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Transduction, Genetic
  • Mice
  • Kidney Tubules, Proximal
  • Humans
  • Human Immunodeficiency Virus Proteins
  • HIV-1
  • Giant Cells
  • Gene Products, vpr
  • Epithelial Cells