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Novel function of prothymosin alpha as a potent inhibitor of human immunodeficiency virus type 1 gene expression in primary macrophages.

Publication ,  Journal Article
Mosoian, A; Teixeira, A; High, AA; Christian, RE; Hunt, DF; Shabanowitz, J; Liu, X; Klotman, M
Published in: J Virol
September 2006

CD8(+) T lymphocytes control human immunodeficiency virus type 1 (HIV-1) infection by a cytotoxic major histocompatibility complex-restricted pathway as well as by secretion of noncytotoxic soluble inhibitory factors. Several components of CD8(+) cell supernatants have been identified that contribute to the latter activity. In this study we report that prothymosin alpha (ProTalpha), a protein found in the cell culture medium of the herpesvirus saimiri-transformed CD8(+) T-cell line, K#1 50K, has potent HIV-1-inhibitory activity. Depletion of native ProTalpha from an HIV-1-inhibitory fraction of CD8(+) cell supernatants removes the inhibitory activity, supporting its role in inhibition via soluble mediators. ProTalpha is an abundant, acidic peptide that has been reported to be localized in the nucleus and associated with cell proliferation and activation of transcription. In this report we demonstrate that ProTalpha suppresses HIV-1 replication, its activity is target cell specific, and inhibition occurs following viral integration. Native and recombinant ProTalpha protein potently inhibit HIV-1 long terminal repeat (LTR)-driven gene expression in macrophages. Furthermore studies using different promoters in lentiviral vectors (cytomegalovirus and phosphoglycerate kinase) revealed that suppression of viral replication by ProTalpha is not HIV LTR specific.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

September 2006

Volume

80

Issue

18

Start / End Page

9200 / 9206

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Thymosin
  • Protein Precursors
  • Promoter Regions, Genetic
  • Macrophages
  • Lentivirus
  • Humans
  • Hela Cells
  • HeLa Cells
 

Citation

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Mosoian, A., Teixeira, A., High, A. A., Christian, R. E., Hunt, D. F., Shabanowitz, J., … Klotman, M. (2006). Novel function of prothymosin alpha as a potent inhibitor of human immunodeficiency virus type 1 gene expression in primary macrophages. J Virol, 80(18), 9200–9206. https://doi.org/10.1128/JVI.00589-06
Mosoian, Arevik, Avelino Teixeira, Anthony A. High, Robert E. Christian, Donald F. Hunt, Jeffrey Shabanowitz, Xinyan Liu, and Mary Klotman. “Novel function of prothymosin alpha as a potent inhibitor of human immunodeficiency virus type 1 gene expression in primary macrophages.J Virol 80, no. 18 (September 2006): 9200–9206. https://doi.org/10.1128/JVI.00589-06.
Mosoian A, Teixeira A, High AA, Christian RE, Hunt DF, Shabanowitz J, et al. Novel function of prothymosin alpha as a potent inhibitor of human immunodeficiency virus type 1 gene expression in primary macrophages. J Virol. 2006 Sep;80(18):9200–6.
Mosoian, Arevik, et al. “Novel function of prothymosin alpha as a potent inhibitor of human immunodeficiency virus type 1 gene expression in primary macrophages.J Virol, vol. 80, no. 18, Sept. 2006, pp. 9200–06. Pubmed, doi:10.1128/JVI.00589-06.
Mosoian A, Teixeira A, High AA, Christian RE, Hunt DF, Shabanowitz J, Liu X, Klotman M. Novel function of prothymosin alpha as a potent inhibitor of human immunodeficiency virus type 1 gene expression in primary macrophages. J Virol. 2006 Sep;80(18):9200–9206.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

September 2006

Volume

80

Issue

18

Start / End Page

9200 / 9206

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Thymosin
  • Protein Precursors
  • Promoter Regions, Genetic
  • Macrophages
  • Lentivirus
  • Humans
  • Hela Cells
  • HeLa Cells