Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection.

Published

Journal Article

Defining and preserving the innate antiviral activity found in cervicovaginal secretions is critical. Cervicovaginal lavage (CVL) samples were obtained from 20 healthy women and evaluated for anti-herpes simplex virus (HSV) activity. CVL samples reduced HSV-2 yields by 23-fold (median), and the anti-HSV activity of CVL samples correlated with the concentration of human neutrophil peptides (HNP)-1-3. Both CVL samples and HNP-1-3 interacted with virus and prevented entry after binding. Substantially less protective activity was observed in CVL samples obtained from 20 human immunodeficiency virus--infected subjects, but the addition of CVL samples from healthy subjects enhanced the antiviral activity. The significance of the innate activity was further demonstrated by showing that CVL samples prevented murine genital herpes. Fourteen of 15 mice were protected from genital herpes if they were challenged with HSV-2 pretreated with CVL samples from healthy subjects. In contrast, all 15 mice challenged with untreated HSV-2 died. These findings are evidence that cervicovaginal secretions contribute to innate resistance to HSV-2 and identify defensins as contributors to this activity.

Full Text

Duke Authors

Cited Authors

  • John, M; Keller, MJ; Fam, EH; Cheshenko, N; Hogarty, K; Kasowitz, A; Wallenstein, S; Carlucci, MJ; Tuyama, AC; Lu, W; Klotman, ME; Lehrer, RI; Herold, BC

Published Date

  • November 2005

Published In

Volume / Issue

  • 192 / 10

Start / End Page

  • 1731 - 1740

PubMed ID

  • 16235171

Pubmed Central ID

  • 16235171

Electronic International Standard Serial Number (EISSN)

  • 1537-6613

International Standard Serial Number (ISSN)

  • 1537-6613

Digital Object Identifier (DOI)

  • 10.1086/497168

Language

  • eng