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Lentiviral gene transduction of kidney.

Publication ,  Journal Article
Gusella, GL; Fedorova, E; Hanss, B; Marras, D; Klotman, ME; Klotman, PE
Published in: Hum Gene Ther
February 10, 2002

Gene transfer into kidney holds great potential as a novel therapeutic approach. We have studied the transduction of kidney in vivo after delivery of lentiviral vectors by various routes of administration. A lentiviral vector expressing the bacterial lacZ gene from the cytomegalovirus early promoter was used. The lentiviral vector was delivered into the kidneys of BALB/c mice by retrograde infusion into the ureter, by injection into the renal vein or artery, or by direct injection into the renal parenchyma. Expression of the reporter gene was achieved independently of the route of administration, although it appeared more efficient after parenchymal or ureteral administration. After parenchymal or ureteral infusion, expression of the transgene was localized to the outer medulla and corticomedullary junction. In the case of parenchymal injection, expression of the reporter gene extended to the cortex. Detection of the transgene in the renal proximal tubules was confirmed by in situ polymerase chain reaction after parenchymal or ureteral infusion. On delivery of the lentiviral vector through the renal artery or vein, expression of the reporter gene was markedly lower than was observed with parenchymal or ureteral infusion and was limited to the inner medullary collecting ducts. No apparent histological abnormality was observed after virus administration and transgene expression was stable for at least 3 months. These results provide the first evidence that lentiviral vectors can stably transduce renal cells in vivo and may be effective vehicles for gene delivery to the kidney.

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Published In

Hum Gene Ther

DOI

ISSN

1043-0342

Publication Date

February 10, 2002

Volume

13

Issue

3

Start / End Page

407 / 414

Location

United States

Related Subject Headings

  • Ureter
  • Transduction, Genetic
  • Renal Veins
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lentivirus
  • Kidney
  • Injections, Intra-Arterial
  • Genetic Vectors
 

Citation

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Gusella, G. L., Fedorova, E., Hanss, B., Marras, D., Klotman, M. E., & Klotman, P. E. (2002). Lentiviral gene transduction of kidney. Hum Gene Ther, 13(3), 407–414. https://doi.org/10.1089/10430340252792530
Gusella, G Luca, Elena Fedorova, Basil Hanss, Daniele Marras, Mary E. Klotman, and Paul E. Klotman. “Lentiviral gene transduction of kidney.Hum Gene Ther 13, no. 3 (February 10, 2002): 407–14. https://doi.org/10.1089/10430340252792530.
Gusella GL, Fedorova E, Hanss B, Marras D, Klotman ME, Klotman PE. Lentiviral gene transduction of kidney. Hum Gene Ther. 2002 Feb 10;13(3):407–14.
Gusella, G. Luca, et al. “Lentiviral gene transduction of kidney.Hum Gene Ther, vol. 13, no. 3, Feb. 2002, pp. 407–14. Pubmed, doi:10.1089/10430340252792530.
Gusella GL, Fedorova E, Hanss B, Marras D, Klotman ME, Klotman PE. Lentiviral gene transduction of kidney. Hum Gene Ther. 2002 Feb 10;13(3):407–414.
Journal cover image

Published In

Hum Gene Ther

DOI

ISSN

1043-0342

Publication Date

February 10, 2002

Volume

13

Issue

3

Start / End Page

407 / 414

Location

United States

Related Subject Headings

  • Ureter
  • Transduction, Genetic
  • Renal Veins
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lentivirus
  • Kidney
  • Injections, Intra-Arterial
  • Genetic Vectors