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Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases.

Publication ,  Journal Article
Mashal, RD; Koontz, J; Sklar, J
Published in: Nat Genet
February 1995

We have explored the application of the bacteriophage resolvases T4 endonuclease VII and T7 endonuclease I for detecting mutations in genomic DNA. Heteroduplex DNA fragments prepared by amplification from DNA containing known mutations were cleaved by one or both enzymes at nucleotide mismatches created by 3 of 3 short deletions and 13 of 14 point mutations in fragments as large as 940 basepairs. Heteroduplexes representing all four classes of possible single nucleotide mismatches were cleaved, and the sizes of the cleavage products generated correlated with the location of the mutation. We conclude that bacteriophage resolvases may be useful reagents for the rapid screening of DNA for mutations.

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Published In

Nat Genet

DOI

ISSN

1061-4036

Publication Date

February 1995

Volume

9

Issue

2

Start / End Page

177 / 183

Location

United States

Related Subject Headings

  • Nucleic Acid Heteroduplexes
  • Mutation
  • Molecular Sequence Data
  • Methods
  • Humans
  • Endodeoxyribonucleases
  • Developmental Biology
  • Deoxyribonuclease I
  • DNA Mutational Analysis
  • DNA
 

Citation

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Mashal, R. D., Koontz, J., & Sklar, J. (1995). Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases. Nat Genet, 9(2), 177–183. https://doi.org/10.1038/ng0295-177
Mashal, R. D., J. Koontz, and J. Sklar. “Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases.Nat Genet 9, no. 2 (February 1995): 177–83. https://doi.org/10.1038/ng0295-177.
Mashal RD, Koontz J, Sklar J. Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases. Nat Genet. 1995 Feb;9(2):177–83.
Mashal, R. D., et al. “Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases.Nat Genet, vol. 9, no. 2, Feb. 1995, pp. 177–83. Pubmed, doi:10.1038/ng0295-177.
Mashal RD, Koontz J, Sklar J. Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases. Nat Genet. 1995 Feb;9(2):177–183.

Published In

Nat Genet

DOI

ISSN

1061-4036

Publication Date

February 1995

Volume

9

Issue

2

Start / End Page

177 / 183

Location

United States

Related Subject Headings

  • Nucleic Acid Heteroduplexes
  • Mutation
  • Molecular Sequence Data
  • Methods
  • Humans
  • Endodeoxyribonucleases
  • Developmental Biology
  • Deoxyribonuclease I
  • DNA Mutational Analysis
  • DNA