Phosphatases driving mitosis: pushing the gas and lifting the brakes.

Published

Journal Article (Review)

Entry into and progression through mitosis depends critically on the establishment and maintenance of protein phosphorylation. For this reason, studies on mitotic progression have focused heavily on the activation of MPF (M phase promoting factor), a cyclin-dependent kinase responsible for phosphorylating proteins that execute the dynamic events of mitosis. Recent work, however, has significantly expanded our understanding of mechanisms that allow accumulation of phosphoproteins at M phase, suggesting that mitotic entry relies not only on MPF activation but also on the inhibition of antimitotic phosphatases. It is now clear that there exists a separate, albeit equally important, signaling pathway for the inactivation of protein phosphatases at the G2/M transition. This pathway, which is governed by the kinase Greatwall is essential for both entry into and maintenance of M phase. This chapter will outline the molecular events regulating entry into mitosis, specifically highlighting the role that protein phosphorylation plays in triggering both MPF activation and the inhibition of phosphatase activity that would otherwise prevent accumulation of mitotic phosphoproteins. These intricate regulatory pathways are essential for maintaining normal cell division and preventing inappropriate cell proliferation, a central hallmark of cancer cells.

Full Text

Duke Authors

Cited Authors

  • Johnson, ES; Kornbluth, S

Published Date

  • January 2012

Published In

Volume / Issue

  • 106 /

Start / End Page

  • 327 - 341

PubMed ID

  • 22340723

Pubmed Central ID

  • 22340723

Electronic International Standard Serial Number (EISSN)

  • 1878-0814

International Standard Serial Number (ISSN)

  • 1877-1173

Digital Object Identifier (DOI)

  • 10.1016/b978-0-12-396456-4.00008-0

Language

  • eng