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HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.

Publication ,  Journal Article
Sasaki, T; Gan, EC; Wakeham, A; Kornbluth, S; Mak, TW; Okada, H
Published in: Genes & development
April 2007

In response to DNA damage, p53 undergoes post-translational modifications (including acetylation) that are critical for its transcriptional activity. However, the mechanism by which p53 acetylation is regulated is still unclear. Here, we describe an essential role for HLA-B-associated transcript 3 (Bat3)/Scythe in controlling the acetylation of p53 required for DNA damage responses. Depletion of Bat3 from human and mouse cells markedly impairs p53-mediated transactivation of its target genes Puma and p21. Although DNA damage-induced phosphorylation, stabilization, and nuclear accumulation of p53 are not significantly affected by Bat3 depletion, p53 acetylation is almost completely abolished. Bat3 forms a complex with p300, and an increased amount of Bat3 enhances the recruitment of p53 to p300 and facilitates subsequent p53 acetylation. In contrast, Bat3-depleted cells show reduced p53-p300 complex formation and decreased p53 acetylation. Furthermore, consistent with our in vitro findings, thymocytes from Bat3-deficient mice exhibit reduced induction of puma and p21, and are resistant to DNA damage-induced apoptosis in vivo. Our data indicate that Bat3 is a novel and essential regulator of p53-mediated responses to genotoxic stress, and that Bat3 controls DNA damage-induced acetylation of p53.

Duke Scholars

Published In

Genes & development

DOI

EISSN

1549-5477

ISSN

0890-9369

Publication Date

April 2007

Volume

21

Issue

7

Start / End Page

848 / 861

Related Subject Headings

  • p300-CBP Transcription Factors
  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Transfection
  • Transcription, Genetic
  • Transcription Factors
  • Thymus Gland
  • Proteins
  • Molecular Sequence Data
  • Molecular Chaperones
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sasaki, T., Gan, E. C., Wakeham, A., Kornbluth, S., Mak, T. W., & Okada, H. (2007). HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53. Genes & Development, 21(7), 848–861. https://doi.org/10.1101/gad.1534107
Sasaki, Toru, Eugene C. Gan, Andrew Wakeham, Sally Kornbluth, Tak W. Mak, and Hitoshi Okada. “HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.Genes & Development 21, no. 7 (April 2007): 848–61. https://doi.org/10.1101/gad.1534107.
Sasaki T, Gan EC, Wakeham A, Kornbluth S, Mak TW, Okada H. HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53. Genes & development. 2007 Apr;21(7):848–61.
Sasaki, Toru, et al. “HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.Genes & Development, vol. 21, no. 7, Apr. 2007, pp. 848–61. Epmc, doi:10.1101/gad.1534107.
Sasaki T, Gan EC, Wakeham A, Kornbluth S, Mak TW, Okada H. HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53. Genes & development. 2007 Apr;21(7):848–861.

Published In

Genes & development

DOI

EISSN

1549-5477

ISSN

0890-9369

Publication Date

April 2007

Volume

21

Issue

7

Start / End Page

848 / 861

Related Subject Headings

  • p300-CBP Transcription Factors
  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Transfection
  • Transcription, Genetic
  • Transcription Factors
  • Thymus Gland
  • Proteins
  • Molecular Sequence Data
  • Molecular Chaperones