Analysis of the cell cycle using Xenopus egg extracts.

Journal Article (Journal Article)

In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.

Full Text

Duke Authors

Cited Authors

  • Kornbluth, S; Yang, J; Powers, M

Published Date

  • January 2006

Published In

Volume / Issue

  • Chapter 11 /

Start / End Page

  • Unit - 11.11

PubMed ID

  • 18228475

Electronic International Standard Serial Number (EISSN)

  • 1934-2616

International Standard Serial Number (ISSN)

  • 1934-2500

Digital Object Identifier (DOI)

  • 10.1002/0471143030.cb1111s29


  • eng