Analysis of the cell cycle using Xenopus egg extracts.

Journal Article (Journal Article)

In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.

Full Text

Published version (via Digital Object Identifier)

Duke Authors

Kornbluth, Sally A.

Cited Authors

Kornbluth, S; Yang, J; Powers, M

Published Date

January 2006

Published In

Current Protocols in Cell Biology

Volume / Issue

Chapter 11 /

Start / End Page

Unit - 11.11

PubMed ID

18228475

Electronic International Standard Serial Number (EISSN)

1934-2616

International Standard Serial Number (ISSN)

1934-2500

Digital Object Identifier (DOI)

10.1002/0471143030.cb1111s29

Language

eng