When the checkpoints have gone: insights into Cdc25 functional activation.

Published

Journal Article

DNA-responsive checkpoints operate at the G(2)/M transition to prevent premature mitosis in the presence of incompletely replicated or damaged DNA. These pathways prevent mitotic entry, at least in part, by suppressing Cdc25, the phosphatase that activates Cdc2/Cyclin B. To gain insight into how checkpoint signaling controls Cdc25 function, we have carefully examined the individual steps in Cdc25 activation. We found that removal of the regulatory protein, 14-3-3, that binds to phosphorylated Cdc25 during interphase is one of the early steps in mitotic activation. Moreover, our studies unexpectedly implicated the phosphatase PP1 and the G(1)/S kinase Cdk2 in the process of Cdc25 activation. Here we integrate our findings and those of others to propose a model for Cdc25 activation in an effort to provide insight into novel loci of DNA-responsive checkpoint control of mitotic entry.

Full Text

Duke Authors

Cited Authors

  • Margolis, SS; Kornbluth, S

Published Date

  • April 2004

Published In

Volume / Issue

  • 3 / 4

Start / End Page

  • 425 - 428

PubMed ID

  • 15020839

Pubmed Central ID

  • 15020839

Electronic International Standard Serial Number (EISSN)

  • 1551-4005

International Standard Serial Number (ISSN)

  • 1538-4101

Language

  • eng