Analysis of apoptosis using Xenopus egg extracts.

Published

Journal Article

In the presence of a subcellular fraction enriched for mitochondria, after prolonged incubation the Xenopus egg extract can mimic biochemical aspects of apoptosis such as caspase activation, and DNA fragmentation. This unit describes preparation of an apoptotic extract from a crude interphase extract and an extract fractionated into latent and execution phases. An apoptotic extract can also be reconstituted from a fractionated interphase extract and purified mitochondria. Protocols are also included for monitoring apoptotic progression in the extract either by following activation of apoptotic proteases (caspases) or by assessing translocation of cytochrome c from the mitochondria to the cytosol.

Full Text

Duke Authors

Cited Authors

  • Kornbluth, S; Evans, EK

Published Date

  • May 2001

Published In

Volume / Issue

  • Chapter 11 /

Start / End Page

  • Unit - 11.12

PubMed ID

  • 18228303

Pubmed Central ID

  • 18228303

Electronic International Standard Serial Number (EISSN)

  • 1934-2616

International Standard Serial Number (ISSN)

  • 1934-2500

Digital Object Identifier (DOI)

  • 10.1002/0471143030.cb1112s09

Language

  • eng