Crk is required for apoptosis in Xenopus egg extracts.

Published

Journal Article

Apoptosis is essential for the development and homeostasis of multicellular organisms. Recently, a cell-free extract prepared from Xenopus eggs was shown to recapitulate intracellular apoptotic pathways in vitro. While many stimuli have been shown to trigger apoptosis in a variety of cell types, the intracellular signaling pathways involved in apoptosis remain largely unknown. Here we show that addition of a recombinant protein containing the phosphotyrosine binding (SH2) domain from the adaptor protein crk, but not those derived from a panel of other signaling proteins, can prevent apoptosis in the Xenopus egg extract system. Furthermore, immunodepletion of endogenous crk protein from the egg extracts, or addition of anti-crk antisera to these extracts, prevents apoptosis. The ability to undergo apoptosis can be restored to these extracts by addition of recombinant crk protein. These results directly demonstrate that crk participates in apoptotic signaling.

Full Text

Duke Authors

Cited Authors

  • Evans, EK; Lu, W; Strum, SL; Mayer, BJ; Kornbluth, S

Published Date

  • January 1997

Published In

Volume / Issue

  • 16 / 2

Start / End Page

  • 230 - 241

PubMed ID

  • 9029144

Pubmed Central ID

  • 9029144

Electronic International Standard Serial Number (EISSN)

  • 1460-2075

International Standard Serial Number (ISSN)

  • 0261-4189

Digital Object Identifier (DOI)

  • 10.1093/emboj/16.2.230

Language

  • eng