Association of p62c-yes with polyomavirus middle T-antigen mutants correlates with transforming ability.

Published

Journal Article

A number of mutants of polyomavirus middle T antigen (MTag) were constructed into replication-competent avian retroviruses. To assess the ability of these MTag variants to transform and to associate with the avian p60c-src and p62c-yes proto-oncogene products, we used these viruses to infect chicken embryo fibroblasts. We found that the ability of individual mutant MTags to associate with p62c-yes correlated well with the ability of these mutants to transform, as has been previously shown for the association of MTag with p60c-src. All transformation-competent mutant MTags retained the ability to complex with p62c-yes. Two transformation-defective mutants, RX67 and RX68, which could weakly associate with p60c-src, were unable to associate with p62c-yes.dl1015, a transformation-defective mutant which could associate with p60c-src and with a phosphatidylinositol kinase activity, was also able to associate with p62c-yes. Therefore, some as yet unmeasured biochemical property is defective in this mutant.

Full Text

Duke Authors

Cited Authors

  • Kornbluth, S; Cheng, SH; Markland, W; Fukui, Y; Hanafusa, H

Published Date

  • April 1990

Published In

Volume / Issue

  • 64 / 4

Start / End Page

  • 1584 - 1589

PubMed ID

  • 1690822

Pubmed Central ID

  • 1690822

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng