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Surfactant protein-A inhibits mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity.

Publication ,  Journal Article
Ledford, JG; Lo, B; Kislan, MM; Thomas, JM; Evans, K; Cain, DW; Kraft, M; Williams, KL; Wright, JR
Published in: J Immunol
October 1, 2010

During pulmonary infections, a careful balance between activation of protective host defense mechanisms and potentially injurious inflammatory processes must be maintained. Surfactant protein A (SP-A) is an immune modulator that increases pathogen uptake and clearance by phagocytes while minimizing lung inflammation by limiting dendritic cell (DC) and T cell activation. Recent publications have shown that SP-A binds to and is bacteriostatic for Mycoplasma pneumoniae in vitro. In vivo, SP-A aids in maintenance of airway homeostasis during M. pneumoniae pulmonary infection by preventing an overzealous proinflammatory response mediated by TNF-α. Although SP-A was shown to inhibit maturation of DCs in vitro, the consequence of DC/SP-A interactions in vivo has not been elucidated. In this article, we show that the absence of SP-A during M. pneumoniae infection leads to increased numbers of mature DCs in the lung and draining lymph nodes during the acute phase of infection and, consequently, increased numbers of activated T and B cells during the course of infection. The findings that glycyrrhizin, a specific inhibitor of extracellular high-mobility group box-1 (HMGB-1) abrogated this effect and that SP-A inhibits HMGB-1 release from immune cells suggest that SP-A inhibits M. pneumoniae-induced DC maturation by regulating HMGB-1 cytokine activity.

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Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

October 1, 2010

Volume

185

Issue

7

Start / End Page

3884 / 3894

Location

United States

Related Subject Headings

  • Pulmonary Surfactant-Associated Protein A
  • Mycoplasma pneumoniae
  • Mycoplasma Infections
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • HMGB1 Protein
  • Flow Cytometry
  • Dendritic Cells
 

Citation

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Chicago
ICMJE
MLA
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Ledford, J. G., Lo, B., Kislan, M. M., Thomas, J. M., Evans, K., Cain, D. W., … Wright, J. R. (2010). Surfactant protein-A inhibits mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity. J Immunol, 185(7), 3884–3894. https://doi.org/10.4049/jimmunol.1000387
Ledford, Julie G., Bernice Lo, Michele M. Kislan, Joseph M. Thomas, Katherine Evans, Derek W. Cain, Monica Kraft, Kristi L. Williams, and Jo Rae Wright. “Surfactant protein-A inhibits mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity.J Immunol 185, no. 7 (October 1, 2010): 3884–94. https://doi.org/10.4049/jimmunol.1000387.
Ledford JG, Lo B, Kislan MM, Thomas JM, Evans K, Cain DW, et al. Surfactant protein-A inhibits mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity. J Immunol. 2010 Oct 1;185(7):3884–94.
Ledford, Julie G., et al. “Surfactant protein-A inhibits mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity.J Immunol, vol. 185, no. 7, Oct. 2010, pp. 3884–94. Pubmed, doi:10.4049/jimmunol.1000387.
Ledford JG, Lo B, Kislan MM, Thomas JM, Evans K, Cain DW, Kraft M, Williams KL, Wright JR. Surfactant protein-A inhibits mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity. J Immunol. 2010 Oct 1;185(7):3884–3894.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

October 1, 2010

Volume

185

Issue

7

Start / End Page

3884 / 3894

Location

United States

Related Subject Headings

  • Pulmonary Surfactant-Associated Protein A
  • Mycoplasma pneumoniae
  • Mycoplasma Infections
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • HMGB1 Protein
  • Flow Cytometry
  • Dendritic Cells