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Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the β-adrenergic receptor

Publication ,  Journal Article
Kraus, WE; Longabaugh, JP; Liggett, SB
Published in: American Journal of Physiology - Endocrinology and Metabolism
January 1, 1992

Continuous electrical pacing (EP) at 10 Hz of the peroneal nerve innervating fast-twitch muscles of the hindlimb in adult rabbits increases skeletal muscle concentrations of adenosine 3',5'-cyclic monophosphate (cAMP) by 3.1-fold at 10 days and increases β-adrenergic receptor (β-AR) density by 2.0-fold at 21 days. To determine whether β-AR, the α-subunit of guanine nucleotide proteins (G(sα)), or adenylyl cyclase is primarily responsible for pacing-induced increases in muscle cAMP, we measured adenylyl cyclase activity (ACA) in muscles that were electrically paced for 3 (n = 4), 10 (n = 8), and 21 (n = 8) days. EP resulted in a time-dependent increase in ACA that was 2.2 ± 0.3-fold (P < 0.005) at 21 days. EP significantly increased GTP-, 5'-guanylylimidodiphosphate-, isoproterenol-, NaF-, and forskolin-stimulated ACA, and propranolol administration to rabbits during EP did not alter pacing-induced changes in ACA. There were no changes in protein concentration, Na+-K+-adenosinetriphosphatase activity, or G(sα) with EP. Based on these studies, we conclude that EP appears to increase cAMP through mechanisms independent of the β-AR and through mechanisms that may involve alterations at the level of adenylyl cyclase.

Duke Scholars

Published In

American Journal of Physiology - Endocrinology and Metabolism

ISSN

0002-9513

Publication Date

January 1, 1992

Volume

263

Issue

2 26-2

Related Subject Headings

  • Cardiovascular System & Hematology
 

Citation

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Kraus, W. E., Longabaugh, J. P., & Liggett, S. B. (1992). Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the β-adrenergic receptor. American Journal of Physiology - Endocrinology and Metabolism, 263(2 26-2).
Kraus, W. E., J. P. Longabaugh, and S. B. Liggett. “Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the β-adrenergic receptor.” American Journal of Physiology - Endocrinology and Metabolism 263, no. 2 26-2 (January 1, 1992).
Kraus WE, Longabaugh JP, Liggett SB. Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the β-adrenergic receptor. American Journal of Physiology - Endocrinology and Metabolism. 1992 Jan 1;263(2 26-2).
Kraus, W. E., et al. “Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the β-adrenergic receptor.” American Journal of Physiology - Endocrinology and Metabolism, vol. 263, no. 2 26-2, Jan. 1992.
Kraus WE, Longabaugh JP, Liggett SB. Electrical pacing induces adenylyl cyclase in skeletal muscle independent of the β-adrenergic receptor. American Journal of Physiology - Endocrinology and Metabolism. 1992 Jan 1;263(2 26-2).

Published In

American Journal of Physiology - Endocrinology and Metabolism

ISSN

0002-9513

Publication Date

January 1, 1992

Volume

263

Issue

2 26-2

Related Subject Headings

  • Cardiovascular System & Hematology