This article reviews several contemporary molecular approaches, both genetic and immunologic, in laboratory diagnostics, emphasizing underlying technologic principles, basic methodologies, and some relevant examples of clinical applicability. Specific methods discussed include Southern, Northern, dot, and in situ hybridization; S nuclease and RNase protection assays; polymerase chain reaction; reverse transcription; and ELISA.
Hardy, KJ; Young, HA; Lagoo, AS
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