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Genomic structure and chromosomal localization of the novel ETS factor, PE-2 (ERF).

Publication ,  Journal Article
de Castro, CM; Rabe, SM; Langdon, SD; Fleenor, DE; Slentz-Kesler, K; Ahmed, MN; Qumsiyeh, MB; Kaufman, RE
Published in: Genomics
June 1, 1997

The members of the ETS family of transcription factors are grouped because they share a highly conserved DNA binding domain. These factors are involved in growth factor pathways and regulate both proliferation and differentiation. To identify ETS factors that may be involved in early hematopoietic progenitor regulation, we isolated a novel member of the ETS family by reverse transcriptase-PCR of the conserved DNA binding domain using degenerate oligonucleotides. This gene directs the synthesis of a 2704-nucleotide transcript whose largest open reading frame encodes a 548-amino-acid protein. Northern blot analysis reveals ubiquitous expression in all human tissues and cell lines tested, with highest levels in the testis, ovary, pancreas, and heart. Comparison of this gene with the available databases reveals very significant homology to the ETS factor PE-1 and probable near-identity with the recently cloned factor ERF. The PE-2 gene is composed of four exons spanning over 9 kb of genomic DNA. Sequence analysis of the promoter region reveals a GC-rich sequence without a TATA motif and with putative binding motifs for CREB, c-myb, and AP-1 factors. Using mouse-human somatic hybrids and FISH analysis, the PE-2 gene is localized to human chromosome 19q13.2, a region involved in translocations and deletions in leukemias and several solid tumors, suggesting that this novel ETS factor may play a role in carcinogenesis.

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Published In

Genomics

DOI

ISSN

0888-7543

Publication Date

June 1, 1997

Volume

42

Issue

2

Start / End Page

227 / 235

Location

United States

Related Subject Headings

  • Transcription Factors
  • Tissue Distribution
  • Repressor Proteins
  • RNA
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • Mice
  • Male
  • In Situ Hybridization, Fluorescence
 

Citation

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de Castro, C. M., Rabe, S. M., Langdon, S. D., Fleenor, D. E., Slentz-Kesler, K., Ahmed, M. N., … Kaufman, R. E. (1997). Genomic structure and chromosomal localization of the novel ETS factor, PE-2 (ERF). Genomics, 42(2), 227–235. https://doi.org/10.1006/geno.1997.4730
Castro, C. M. de, S. M. Rabe, S. D. Langdon, D. E. Fleenor, K. Slentz-Kesler, M. N. Ahmed, M. B. Qumsiyeh, and R. E. Kaufman. “Genomic structure and chromosomal localization of the novel ETS factor, PE-2 (ERF).Genomics 42, no. 2 (June 1, 1997): 227–35. https://doi.org/10.1006/geno.1997.4730.
de Castro CM, Rabe SM, Langdon SD, Fleenor DE, Slentz-Kesler K, Ahmed MN, et al. Genomic structure and chromosomal localization of the novel ETS factor, PE-2 (ERF). Genomics. 1997 Jun 1;42(2):227–35.
de Castro, C. M., et al. “Genomic structure and chromosomal localization of the novel ETS factor, PE-2 (ERF).Genomics, vol. 42, no. 2, June 1997, pp. 227–35. Pubmed, doi:10.1006/geno.1997.4730.
de Castro CM, Rabe SM, Langdon SD, Fleenor DE, Slentz-Kesler K, Ahmed MN, Qumsiyeh MB, Kaufman RE. Genomic structure and chromosomal localization of the novel ETS factor, PE-2 (ERF). Genomics. 1997 Jun 1;42(2):227–235.
Journal cover image

Published In

Genomics

DOI

ISSN

0888-7543

Publication Date

June 1, 1997

Volume

42

Issue

2

Start / End Page

227 / 235

Location

United States

Related Subject Headings

  • Transcription Factors
  • Tissue Distribution
  • Repressor Proteins
  • RNA
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • Mice
  • Male
  • In Situ Hybridization, Fluorescence