Comparison of human and Xenopus GATA-2 promoters.
Journal Article (Journal Article)
GATA proteins comprise a family of transcription factors that are required for appropriate development of hematopoietic lineages. In order to understand the transcriptional regulation of GATA genes, we have cloned the human GATA-2 gene and identified and characterized its promoter. Comparison with the Xenopus GATA-2 promoter demonstrates highly conserved CCAAT box elements, which are essential for appropriate Xenopus expression. Unlike the Xenopus gene, the human GATA-2 gene lacks GATA binding motifs within the first 800 bp of 5' flanking sequence. In addition, the human GATA-2 promoter has two highly conserved ets sites that resemble the binding site for a recently described ets repressor factor, ERF. These conserved DNA sequence motifs provide strong candidate regions for the regulation of GATA-2.
Full Text
Duke Authors
Cited Authors
- Fleenor, DE; Langdon, SD; deCastro, CM; Kaufman, RE
Published Date
- November 14, 1996
Published In
Volume / Issue
- 179 / 2
Start / End Page
- 219 - 223
PubMed ID
- 8972903
International Standard Serial Number (ISSN)
- 0378-1119
Digital Object Identifier (DOI)
- 10.1016/s0378-1119(96)00355-1
Language
- eng
Conference Location
- Netherlands