Comparison of human and Xenopus GATA-2 promoters.

Journal Article

GATA proteins comprise a family of transcription factors that are required for appropriate development of hematopoietic lineages. In order to understand the transcriptional regulation of GATA genes, we have cloned the human GATA-2 gene and identified and characterized its promoter. Comparison with the Xenopus GATA-2 promoter demonstrates highly conserved CCAAT box elements, which are essential for appropriate Xenopus expression. Unlike the Xenopus gene, the human GATA-2 gene lacks GATA binding motifs within the first 800 bp of 5' flanking sequence. In addition, the human GATA-2 promoter has two highly conserved ets sites that resemble the binding site for a recently described ets repressor factor, ERF. These conserved DNA sequence motifs provide strong candidate regions for the regulation of GATA-2.

Full Text

Duke Authors

Cited Authors

  • Fleenor, DE; Langdon, SD; deCastro, CM; Kaufman, RE

Published Date

  • November 14, 1996

Published In

Volume / Issue

  • 179 / 2

Start / End Page

  • 219 - 223

PubMed ID

  • 8972903

International Standard Serial Number (ISSN)

  • 0378-1119

Language

  • eng

Conference Location

  • Netherlands