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In vitro functional testing of endothelial progenitor cells that overexpress thrombomodulin.

Publication ,  Journal Article
Stroncek, JD; Xue, Y; Haque, N; Lawson, JH; Reichert, WM
Published in: Tissue Eng Part A
August 2011

This study investigated the augmentation of endothelial progenitor cell (EPC) thromboresistance by using gene therapy to overexpress thrombomodulin (TM), an endothelial cell membrane glycoprotein that has potent anti-coagulant properties. Late outgrowth EPCs were isolated from peripheral blood of patients with documented coronary artery disease and transfected with an adenoviral vector containing human TM. EPC transfection conditions for maximizing TM expression, transfection efficiency, and cell viability were employed. TM-overexpressing EPCs had a fivefold increase in the rate of activated protein C production over native EPCs and EPCs transfected with an adenoviral control vector expressing β-galactosidase (p<0.05). TM upregulation caused a significant threefold reduction in platelet adhesion compared to native EPCs, and a 12-fold reduction compared to collagen I-coated wells. Additionally, the clotting time of TM-transfected EPCs incubated with whole blood was significantly extended by 19% over native cells (p<0.05). These data indicate that TM-overexpression has the potential to improve the antithrombotic performance of patient-derived EPCs for endothelialization applications.

Duke Scholars

Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

August 2011

Volume

17

Issue

15-16

Start / End Page

2091 / 2100

Location

United States

Related Subject Headings

  • Transfection
  • Tissue Engineering
  • Time Factors
  • Thrombomodulin
  • Stem Cells
  • Rheology
  • Protein C
  • Platelet Adhesiveness
  • Humans
  • Endothelial Cells
 

Citation

APA
Chicago
ICMJE
MLA
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Stroncek, J. D., Xue, Y., Haque, N., Lawson, J. H., & Reichert, W. M. (2011). In vitro functional testing of endothelial progenitor cells that overexpress thrombomodulin. Tissue Eng Part A, 17(15–16), 2091–2100. https://doi.org/10.1089/ten.TEA.2010.0631
Stroncek, John D., Yujing Xue, Nabila Haque, Jeffrey H. Lawson, and William M. Reichert. “In vitro functional testing of endothelial progenitor cells that overexpress thrombomodulin.Tissue Eng Part A 17, no. 15–16 (August 2011): 2091–2100. https://doi.org/10.1089/ten.TEA.2010.0631.
Stroncek JD, Xue Y, Haque N, Lawson JH, Reichert WM. In vitro functional testing of endothelial progenitor cells that overexpress thrombomodulin. Tissue Eng Part A. 2011 Aug;17(15–16):2091–100.
Stroncek, John D., et al. “In vitro functional testing of endothelial progenitor cells that overexpress thrombomodulin.Tissue Eng Part A, vol. 17, no. 15–16, Aug. 2011, pp. 2091–100. Pubmed, doi:10.1089/ten.TEA.2010.0631.
Stroncek JD, Xue Y, Haque N, Lawson JH, Reichert WM. In vitro functional testing of endothelial progenitor cells that overexpress thrombomodulin. Tissue Eng Part A. 2011 Aug;17(15–16):2091–2100.

Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

August 2011

Volume

17

Issue

15-16

Start / End Page

2091 / 2100

Location

United States

Related Subject Headings

  • Transfection
  • Tissue Engineering
  • Time Factors
  • Thrombomodulin
  • Stem Cells
  • Rheology
  • Protein C
  • Platelet Adhesiveness
  • Humans
  • Endothelial Cells